Quantification of Brucella abortus population structure in a natural host.

Autor:	Fiebig A; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824., Vrentas CE; Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, IA 50010., Le T; Department of Statistics and Probability, Michigan State University, East Lansing, MI 48824., Huebner M; Department of Statistics and Probability, Michigan State University, East Lansing, MI 48824., Boggiatto PM; Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, IA 50010., Olsen SC; Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, IA 50010., Crosson S; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824; crosson4@msu.edu.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Mar 16; Vol. 118 (11).
DOI:	10.1073/pnas.2023500118
Abstrakt:	Cattle are natural hosts of the intracellular pathogen Brucella abortus , which inflicts a significant burden on the health and reproduction of these important livestock. The primary routes of infection in field settings have been described, but it is not known how the bovine host shapes the structure of B. abortus populations during infection. We utilized a library of uniquely barcoded B. abortus strains to temporally and spatially quantify population structure during colonization of cattle through a natural route of infection. Introducing 10 8 bacteria from this barcoded library to the conjunctival mucosa resulted in expected levels of local lymph node colonization at a 1-wk time point. We leveraged variance in strain abundance in the library to demonstrate that only 1 in 10,000 brucellae introduced at the site of infection reached a parotid lymph node. Thus, cattle restrict the overwhelming majority of B. abortus introduced via the ocular conjunctiva at this dose. Individual strains were spatially restricted within the host tissue, and the total B. abortus census was dominated by a small number of distinct strains in each lymph node. These results define a bottleneck that B. abortus must traverse to colonize local lymph nodes from the conjunctival mucosa. The data further support a model in which a small number of spatially isolated granulomas founded by unique strains are present at 1 wk postinfection. These experiments demonstrate the power of barcoded transposon tools to quantify infection bottlenecks and to define pathogen population structure in host tissues. Competing Interests: The authors declare no competing interest.
Databáze:	MEDLINE
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