Enhancement of the antibiotic activity by quercetin against Staphylococcus aureus efflux pumps.

Autor: Dos Santos JFS; Laboratory of Semi-Arid Bioprospecting (Lab-Bioprospec), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil., Tintino SR; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil., da Silva ARP; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil., Dos S Barbosa CR; Laboratory of Semi-Arid Bioprospecting (Lab-Bioprospec), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil., Scherf JR; Laboratory of Semi-Arid Bioprospecting (Lab-Bioprospec), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil., de S Silveira Z; Laboratory of Semi-Arid Bioprospecting (Lab-Bioprospec), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil., de Freitas TS; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil., de Lacerda Neto LJ; Laboratory of Semi-Arid Bioprospecting (Lab-Bioprospec), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil., Barros LM; Laboratory of Semi-Arid Bioprospecting (Lab-Bioprospec), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil., de A Menezes IR; Laboratory of Pharmacology and Molecular Chemistry (LFQM), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil., Coutinho HDM; Laboratory of Microbiology and Molecular Biology (LMBM), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil. hdmcoutinho@gmail.com.; Department of Biological Chemistry, Microbiology and Molecular Biology Laboratory (LMBM), Regional University of Cariri, DQB/ URCA, Av. Cel. Antonio Luiz, 1161. Pimenta, Crato, Ceará, CEP: 63105-000, Brazil. hdmcoutinho@gmail.com., Siqueira-Júnior JP; Department of Molecular Biology, Federal University of Paraíba, João Pessoa, Paraíba, CEP: 58051-900, Brazil., Cunha FAB; Laboratory of Semi-Arid Bioprospecting (Lab-Bioprospec), Department of Biological Chemistry, DQB/URCA, Crato, Ceará, Brazil.
Jazyk: angličtina
Zdroj: Journal of bioenergetics and biomembranes [J Bioenerg Biomembr] 2021 Apr; Vol. 53 (2), pp. 157-167. Date of Electronic Publication: 2021 Mar 08.
DOI: 10.1007/s10863-021-09886-4
Abstrakt: The objective of this work was to evaluate the inhibitory effect of quercetin on S. aureus Efflux Pumps. The MIC of Quercetin was evaluated through the broth microdilution method, as well as the Efflux Pump inhibition assay through the method of reducing the antibiotic minimum inhibitory concentration as well as that of ethidium bromide. The in silico approach through bioinformatics was performed to demonstrate the molecular mechanism of interaction of the substrate and the binding cavity. The Quercetin inhibition concentration was not clinically relevant. With respect to the reversal of bacterial resistance effect by efflux pump inhibition, this effect was observed with the strains carrying the TetK and NorA pumps. Regarding the interaction between the Quercetin complex and the NorA pump, the extra stability was provided by hydrogen bonds produced by the hydroxyl group.
Databáze: MEDLINE
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