Local genetic context shapes the function of a gene regulatory network.
| Autor: | Nagy-Staron A; Institute of Science and Technology Austria, Klosterneuburg, Austria., Tomasek K; Institute of Science and Technology Austria, Klosterneuburg, Austria., Caruso Carter C; Institute of Science and Technology Austria, Klosterneuburg, Austria., Sonnleitner E; Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, Center Of Molecular Biology, University of Vienna, Vienna, Austria., Kavčič B; Institute of Science and Technology Austria, Klosterneuburg, Austria., Paixão T; Institute of Science and Technology Austria, Klosterneuburg, Austria., Guet CC; Institute of Science and Technology Austria, Klosterneuburg, Austria. |
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| Jazyk: | angličtina |
| Zdroj: | ELife [Elife] 2021 Mar 08; Vol. 10. Date of Electronic Publication: 2021 Mar 08. |
| DOI: | 10.7554/eLife.65993 |
| Abstrakt: | Gene expression levels are influenced by multiple coexisting molecular mechanisms. Some of these interactions such as those of transcription factors and promoters have been studied extensively. However, predicting phenotypes of gene regulatory networks (GRNs) remains a major challenge. Here, we use a well-defined synthetic GRN to study in Escherichia coli how network phenotypes depend on local genetic context, i.e. the genetic neighborhood of a transcription factor and its relative position. We show that one GRN with fixed topology can display not only quantitatively but also qualitatively different phenotypes, depending solely on the local genetic context of its components. Transcriptional read-through is the main molecular mechanism that places one transcriptional unit (TU) within two separate regulons without the need for complex regulatory sequences. We propose that relative order of individual TUs, with its potential for combinatorial complexity, plays an important role in shaping phenotypes of GRNs. Competing Interests: AN, KT, CC, ES, BK, TP, CG No competing interests declared (© 2021, Nagy-Staron et al.) |
| Databáze: | MEDLINE |
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