Memory B-Cell Responses Against Merozoite Antigens After Acute Plasmodium falciparum Malaria, Assessed Over One Year Using a Novel Multiplexed FluoroSpot Assay.

Autor: Jahnmatz P; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, Sweden.; Mabtech AB, Nacka Strand, Sweden., Sundling C; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden., Yman V; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, Sweden., Widman L; Division of Biostatistics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden., Asghar M; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden., Sondén K; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden., Stenström C; Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden., Smedman C; Mabtech AB, Nacka Strand, Sweden., Ndungu F; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, Sweden.; Kenya Medical Research Institute (KEMRI)/Wellcome Trust Research Programme, Kilifi, Kenya., Ahlborg N; Mabtech AB, Nacka Strand, Sweden.; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden., Färnert A; Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden.
Jazyk: angličtina
Zdroj: Frontiers in immunology [Front Immunol] 2021 Feb 12; Vol. 11, pp. 619398. Date of Electronic Publication: 2021 Feb 12 (Print Publication: 2020).
DOI: 10.3389/fimmu.2020.619398
Abstrakt: Memory B cells (MBCs) are believed to be important for the maintenance of immunity to malaria, and these cells need to be explored in the context of different parasite antigens and their breadth and kinetics after natural infections. However, frequencies of antigen-specific MBCs are low in peripheral blood, limiting the number of antigens that can be studied, especially when small blood volumes are available. Here, we developed a multiplexed reversed B-cell FluoroSpot assay capable of simultaneously detecting MBCs specific for the four Plasmodium falciparum blood-stage antigens, MSP-1 19 , MSP-2, MSP-3 and AMA-1. We used the assay to study the kinetics of the MBC response after an acute episode of malaria and up to one year following treatment in travelers returning to Sweden from sub-Saharan Africa. We show that the FluoroSpot assay can detect MBCs to all four merozoite antigens in the same well, and that the breadth and kinetics varied between individuals. We further found that individuals experiencing a primary infection could mount and maintain parasite-specific MBCs to a similar extent as previously exposed adults, already after a single infection. We conclude that the multiplexed B-cell FluoroSpot is a powerful tool for assessing antigen-specific MBC responses to several antigens simultaneously, and that the kinetics of MBC responses against merozoite surface antigens differ over the course of one year. These findings contribute to the understanding of acquisition and maintenance of immune responses to malaria.
Competing Interests: PJ, CSm, and NA are employed by Mabtech AB, Sweden. Several of the reagents and the FluoroSpot reader system used in this study are produced by Mabtech. Mabtech has no influence on the content of this study. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Jahnmatz, Sundling, Yman, Widman, Asghar, Sondén, Stenström, Smedman, Ndungu, Ahlborg and Färnert.)
Databáze: MEDLINE
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