Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process.

Autor:	Petre B; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.; Université de Lorraine, INRAE, IAM, Nancy, France., Contreras MP; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK., Bozkurt TO; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.; Department of Life Sciences, Imperial College London, London, UK., Schattat MH; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.; Department of Plant Physiology, Institute for Biology, Martin-Luther University Halle-Wittenberg, Halle, Germany., Sklenar J; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK., Schornack S; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.; Sainsbury Laboratory, University of Cambridge, Cambridge, UK., Abd-El-Haliem A; Phytopathology Research, Rijk Zwaan Breeding BV, Fijnaart, The Netherlands., Castells-Graells R; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.; Molecular Biology Institute, University of California Los Angeles, Los Angeles, California, USA., Lozano-Durán R; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.; Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China., Dagdas YF; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, Vienna, Austria., Menke FLH; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK., Jones AME; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.; School of Life Sciences, University of Warwick, Coventry, UK., Vossen JH; Plant Breeding, Wageningen University and Research, Wageningen, The Netherlands., Robatzek S; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.; Ludwig-Maximilian-University of Munich, Munich, Germany., Kamoun S; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK., Win J; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, UK.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2021 Jul 02; Vol. 33 (5), pp. 1447-1471.
DOI:	10.1093/plcell/koab069
Abstrakt:	Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically function by associating with host molecules and modulating their activities. This study aimed to identify the host processes targeted by the RXLR class of host-translocated effectors of the potato blight pathogen Phytophthora infestans. To this end, we performed an in planta protein-protein interaction screen by transiently expressing P. infestans RXLR effectors in Nicotiana benthamiana leaves followed by coimmunoprecipitation and liquid chromatography-tandem mass spectrometry. This screen generated an effector-host protein interactome matrix of 59 P. infestans RXLR effectors x 586 N. benthamiana proteins. Classification of the host interactors into putative functional categories revealed over 35 biological processes possibly targeted by P. infestans. We further characterized the PexRD12/31 family of RXLR-WY effectors, which associate and colocalize with components of the vesicle trafficking machinery. One member of this family, PexRD31, increased the number of FYVE positive vesicles in N. benthamiana cells. FYVE positive vesicles also accumulated in leaf cells near P. infestans hyphae, indicating that the pathogen may enhance endosomal trafficking during infection. This interactome dataset will serve as a useful resource for functional studies of P. infestans effectors and of effector-targeted host processes. (© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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