Engineered glycomaterial implants orchestrate large-scale functional repair of brain tissue chronically after severe traumatic brain injury.

Autor:	Latchoumane CV; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA.; Edgar L. Rhodes Center for ADS, College of Agriculture and Environmental Sciences, University of Georgia, Athens, GA 30602, USA., Betancur MI; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, 101 Science Drive, Durham, NC 27705, USA., Simchick GA; Department of Physics and Astronomy, University of Georgia, Athens, GA 30602, USA.; Bio-Imaging Research Center, University of Georgia, Athens, GA 30602, USA., Sun MK; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA.; Division of Neuroscience, Biomedical & Health Sciences Institute, University of Georgia, Athens, GA 30602, USA., Forghani R; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA., Lenear CE; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA.; Edgar L. Rhodes Center for ADS, College of Agriculture and Environmental Sciences, University of Georgia, Athens, GA 30602, USA., Ahmed A; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA.; Edgar L. Rhodes Center for ADS, College of Agriculture and Environmental Sciences, University of Georgia, Athens, GA 30602, USA., Mohankumar R; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA., Balaji N; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA., Mason HD; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA., Archer-Hartmann SA; Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA., Azadi P; Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA., Holmes PV; Division of Neuroscience, Biomedical & Health Sciences Institute, University of Georgia, Athens, GA 30602, USA.; Psychology Department, University of Georgia, Athens, GA 30602, USA., Zhao Q; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA.; Department of Physics and Astronomy, University of Georgia, Athens, GA 30602, USA.; Bio-Imaging Research Center, University of Georgia, Athens, GA 30602, USA., Bellamkonda RV; Department of Biomedical Engineering, Pratt School of Engineering, Duke University, 101 Science Drive, Durham, NC 27705, USA., Karumbaiah L; Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA. lohitash@uga.edu.; Edgar L. Rhodes Center for ADS, College of Agriculture and Environmental Sciences, University of Georgia, Athens, GA 30602, USA.; Division of Neuroscience, Biomedical & Health Sciences Institute, University of Georgia, Athens, GA 30602, USA.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2021 Mar 05; Vol. 7 (10). Date of Electronic Publication: 2021 Mar 05 (Print Publication: 2021).
DOI:	10.1126/sciadv.abe0207
Abstrakt:	Severe traumatic brain injury (sTBI) survivors experience permanent functional disabilities due to significant volume loss and the brain's poor capacity to regenerate. Chondroitin sulfate glycosaminoglycans (CS-GAGs) are key regulators of growth factor signaling and neural stem cell homeostasis in the brain. However, the efficacy of engineered CS (eCS) matrices in mediating structural and functional recovery chronically after sTBI has not been investigated. We report that neurotrophic factor functionalized acellular eCS matrices implanted into the rat M1 region acutely after sTBI significantly enhanced cellular repair and gross motor function recovery when compared to controls 20 weeks after sTBI. Animals subjected to M2 region injuries followed by eCS matrix implantations demonstrated the significant recovery of "reach-to-grasp" function. This was attributed to enhanced volumetric vascularization, activity-regulated cytoskeleton (Arc) protein expression, and perilesional sensorimotor connectivity. These findings indicate that eCS matrices implanted acutely after sTBI can support complex cellular, vascular, and neuronal circuit repair chronically after sTBI. (Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
Databáze:	MEDLINE
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