Extracts Prepared from a Canadian Toxic Plant Induce Light-Dependent Perinuclear Vacuoles in Human Cells.

Autor: Tuescher JM; Natural Product and Cancer Cell Laboratories, University of Lethbridge, Lethbridge, AB T1K 3M4, Canada., Beck CR; Natural Product and Cancer Cell Laboratories, University of Lethbridge, Lethbridge, AB T1K 3M4, Canada., Spencer L; Department of Physics and Astronomy, University of Lethbridge, Lethbridge AB T1K 3M4, Canada., Yeremy B; Department of Earth, Ocean, Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z4, Canada., Shi Y; Department of Earth, Ocean, Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z4, Canada., Andersen RJ; Department of Earth, Ocean, Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z4, Canada., Golsteyn RM; Natural Product and Cancer Cell Laboratories, University of Lethbridge, Lethbridge, AB T1K 3M4, Canada.
Jazyk: angličtina
Zdroj: Toxins [Toxins (Basel)] 2021 Feb 12; Vol. 13 (2). Date of Electronic Publication: 2021 Feb 12.
DOI: 10.3390/toxins13020138
Abstrakt: We are investigating plant species from the Canadian prairie ecological zone by phenotypic cell assays to discover toxins of biological interest. We provide the first report of the effects of extracts prepared from the shrub Symphoricarpos occidentalis in several human cell lines. S. occidentalis (Caprifoliaceae) extracts are cytotoxic, and, strikingly, treated cells undergo light-dependent vacuolation near the nucleus. The range of irradiation is present in standard ambient light and lies in the visible range (400-700 nm). Vacuolization in treated cells can be induced with specific wavelengths of 408 or 660 nm at 1 J/cm 2 energies. Vacuolated cells show a striking phenotype of a large perinuclear vacuole (nuclear associated vacuole, NAV) that is distinct from vesicles observed by treatment with an autophagy-inducing agent. Treatment with S. occidentalis extracts and light induces an intense lamin A/C signal at the junction of a nuclear vacuole and the nucleus. Further study of S. occidentalis extracts and vacuolation provide chemical tools that may contribute to the understanding of nuclear envelope organization and human cell biology.
Databáze: MEDLINE
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