| Autor: |
Metakovsky E; Department of Biotechnology-Plant Biology, School of Agricultural, Food and Biosystems Engineering, Universidad Politécnica de Madrid, 28040 Madrid, Spain., Pascual L; Department of Biotechnology-Plant Biology, School of Agricultural, Food and Biosystems Engineering, Universidad Politécnica de Madrid, 28040 Madrid, Spain., Vaccino P; Consiglio per la Recerca in Agricultura e l'Analisi dell'Economia Agraria, Research Centre for Cereal and Industrial Crops, 13100 Vercelli, Italy., Melnik V; Vavilov Institute of General Genetics RAS, 117971 Moscow, Russia., Rodriguez-Quijano M; Department of Biotechnology-Plant Biology, School of Agricultural, Food and Biosystems Engineering, Universidad Politécnica de Madrid, 28040 Madrid, Spain., Popovych Y; Department of Genetics and Molecular Biology, National I.I. Mechnikov University, 65058 Odessa, Ukraine., Chebotar S; Department of Genetics and Molecular Biology, National I.I. Mechnikov University, 65058 Odessa, Ukraine., Rogers WJ; Departamento de Biología Aplicada, CIISAS, CIC-BIOLAB, CONICET-INBIOTEC, CRESCA, Facultad de Agronomía, Universidad Nacional del Centro de la Provincia Buenos Aires, 7300 Azul, Provincia de Buenos Aires, Argentina. |
| Abstrakt: |
The Gli-B1 -encoded γ-gliadins and non-coding γ-gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major γ-gliadin, restriction fragment length polymorphism patterns (RFLPs) (three different markers), Gli-B1 -γ-gliadin-pseudogene known SNP markers (Single nucleotide polymorphisms) and sequencing the pseudogene GAG56B . It was discovered that encoded γ-gliadins, with contrasting EM, had similar MWs. However, seven allelic variants (designated from I to VII) differed among them in the other six tests: I (alleles Gli-B1i , k , m , o ), II ( Gli-B1n , q , s ), III ( Gli-B1b ), IV ( Gli-B1e , f , g ), V ( Gli-B1h ), VI ( Gli-B1d ) and VII ( Gli-B1a ). Allele Gli-B1c (variant VIII) was identical to the alleles from group IV in four of the tests. Some tests might show a fine difference between alleles belonging to the same variant. Our results attest in favor of the independent origin of at least seven variants at the Gli-B1 locus that might originate from deeply diverged genotypes of the donor(s) of the B genome in hexaploid wheat and therefore might be called "heteroallelic". The donor's particularities at the Gli-B1 locus might be conserved since that time and decisively contribute to the current high genetic diversity of common wheat. |
