| Autor: |
Petrilli LL; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy.; Department of Onco-Hematology, Cell and Gene Therapy, Ospedale Pediatrico Bambino Gesù (IRCCS), 00146 Rome, Italy., Riccio F; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Giuliani G; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Palma A; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Gargioli C; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Vumbaca S; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Faron M; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Palmieri G; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Pasquini L; Core Facilities, Istituto Superiore di Sanità, 00161 Rome, Italy., Sacco F; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Cesareni G; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy.; Fondazione Santa Lucia Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), 00143 Rome, Italy., Castagnoli L; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy., Fuoco C; Department of Biology, University of Rome 'Tor Vergata', 00133 Rome, Italy. |
| Abstrakt: |
The embryonal rhabdomyosarcoma (eRMS) is a soft tissue sarcoma commonly affecting the head and neck, the extremities and the genitourinary tract. To contribute to revealing the cell types that may originate this tumor, we exploited mass cytometry, a single-cell technique that, by using heavy-metal-tagged antibodies, allows the accurate monitoring of the changes occurring in the mononuclear cell composition of skeletal muscle tissue during tumor development. To this end, we compared cell populations of healthy muscles with those from spatiotemporal-induced eRMS tumors in a mouse model (LSL-Kras G12D/+ ;Tp53 Fl/Fl ) that can be used to develop rhabdomyosarcoma by means of infection with an adenovirus vector expressing Cre (Ad-Cre) recombinase. By monitoring different time points after tumor induction, we were able to analyze tumor progression and composition, identifying fibro/adipogenic progenitors (FAPs) as the cell type that, in this model system, had a pivotal role in tumor development. In vitro studies highlighted that both FAPs and satellite cells (SCs), upon infection with the Ad-Cre, acquired the potential to develop rhabdomyosarcomas when transplanted into immunocompromised mice. However, only infected FAPs had an antigen profile that was similar to embryonal rhabdomyosarcoma cells. Overall, our analysis supports the involvement of FAPs in eRMS development. |
