| Autor: |
Żyżyńska-Galeńska K; Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec,05-552 Magdalenka, Poland., Bernat A; Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec,05-552 Magdalenka, Poland.; Laboratory of Molecular Diagnostics, Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk & Medical University of Gdańsk, 80-307 Gdańsk, Poland., Piliszek A; Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec,05-552 Magdalenka, Poland., Karasiewicz J; Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec,05-552 Magdalenka, Poland., Szablisty E; Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec,05-552 Magdalenka, Poland., Sacharczuk M; Department of Experimental Genomics, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, Poland.; Faculty of Pharmacy with the Laboratory Medicine Division, Department of Pharmacodynamics, Medical University of Warsaw, Centre for Preclinical Research and Technology, 02-097 Warsaw, Poland., Brewińska-Olchowik M; Laboratory of Cytometry, Nencki Institute of Experimental Biology, 02-093 Warsaw, Poland., Bochenek M; Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, 32-083 Balice, Poland., Grabarek J; Faculty of Biology, Medicine and Health, Division of Developmental Biology & Medicine, University of Manchester, Manchester M13 9PT, UK.; Current address: Cancer Research UK Manchester Institute, University of Manchester, Manchester SK10 4TG, UK., Modliński JA; Department of Experimental Embryology, Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec,05-552 Magdalenka, Poland. |
| Abstrakt: |
The phenomenon of the reprogramming of terminally differentiated cells can be achieved by various means, like somatic cell nuclear transfer, cell fusion with a pluripotent cell, or the introduction of pluripotency genes. Here, we present the evidence that somatic cells can attain the expression of pluripotency markers after their introduction into early embryos. Mouse embryonic fibroblasts introduced between blastomeres of cleaving embryos, within two days of in vitro culture, express transcription factors specific to blastocyst lineages, including pluripotency factors. Analysis of donor tissue marker DNA has revealed that the progeny of introduced cells are found in somatic tissues of foetuses and adult chimaeras, providing evidence for cell reprogramming. Analysis of ploidy has shown that in the chimaeras, the progeny of introduced cells are either diploid or tetraploid, the latter indicating cell fusion. The presence of donor DNA in diploid cells from chimaeric embryos proved that the non-fused progeny of introduced fibroblasts persisted in chimaeras, which is evidence of reprogramming by embryonic niche. When adult somatic (cumulus) cells were introduced into early cleavage embryos, the extent of integration was limited and only cell fusion-mediated reprogramming was observed. These results show that both cell fusion and cell interactions with the embryonic niche reprogrammed somatic cells towards pluripotency. |
