Topography quantifications allow for identifying the contribution of parental strains to physical properties of co-cultured biofilms.

Autor:	Hayta EN; Munich School of Bioengineering and Department of Mechanical Engineering, Technical University of Munich, 85748, Garching, Germany.; Center for Protein Assemblies (CPA), Technical University of Munich, Ernst-Otto-Fischer Straße 8, 85748, Garching, Germany., Rickert CA; Munich School of Bioengineering and Department of Mechanical Engineering, Technical University of Munich, 85748, Garching, Germany.; Center for Protein Assemblies (CPA), Technical University of Munich, Ernst-Otto-Fischer Straße 8, 85748, Garching, Germany., Lieleg O; Munich School of Bioengineering and Department of Mechanical Engineering, Technical University of Munich, 85748, Garching, Germany.; Center for Protein Assemblies (CPA), Technical University of Munich, Ernst-Otto-Fischer Straße 8, 85748, Garching, Germany.
Jazyk:	angličtina
Zdroj:	Biofilm [Biofilm] 2021 Feb 06; Vol. 3, pp. 100044. Date of Electronic Publication: 2021 Feb 06 (Print Publication: 2021).
DOI:	10.1016/j.bioflm.2021.100044
Abstrakt:	Most biofilm research has so far focused on investigating biofilms generated by single bacterial strains. However, such single-species biofilms are rare in nature where bacteria typically coexist with other microorganisms. Although, from a biological view, the possible interactions occurring between different bacteria are well studied, little is known about what determines the material properties of a multi-species biofilm. Here, we ask how the co-cultivation of two B. subtilis strains affects certain important biofilm properties such as surface topography and wetting behavior. We find that, even though each daughter colony typically resembles one of the parent colonies in terms of morphology and wetting, it nevertheless exhibits a significantly different surface topography. Yet, this difference is only detectable via a quantitative metrological analysis of the biofilm surface. Furthermore, we show that this difference is due to the presence of bacteria belonging to the 'other' parent strain, which does not dominate the biofilm features. The findings presented here may pinpoint new strategies for how biofilms with hybrid properties could be generated from two different bacterial strains. In such engineered biofilms, it might be possible to combine desired properties from two strains by co-cultivation. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Authors.)
Databáze:	MEDLINE
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