Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model.

Autor: Baloche V; CNRS, UMR 9018, Gustave Roussy and Université Paris-Saclay, 39 Rue Camille Desmoulins, F-94805, Villejuif, France., Rivière J; Inserm, U1170, Gustave Roussy, 39 Rue Camille Desmoulins, F-94805, Villejuif, France., Tran TBT; CNRS, UMR 9018, Gustave Roussy and Université Paris-Saclay, 39 Rue Camille Desmoulins, F-94805, Villejuif, France., Gelin A; CNRS, UMR 9018, Gustave Roussy and Université Paris-Saclay, 39 Rue Camille Desmoulins, F-94805, Villejuif, France., Bawa O; Plateforme de pathologie expérimentale et translationnelle, UMS AMMICA, Gustave Roussy, 39 Rue Camille Desmoulins, F-94805, Villejuif, France., Signolle N; Plateforme de pathologie expérimentale et translationnelle, UMS AMMICA, Gustave Roussy, 39 Rue Camille Desmoulins, F-94805, Villejuif, France., Diop MBK; Plateforme de Bioinformatique, UMS AMMICA, Gustave Roussy, 39 Rue Camille Desmoulins, F-94805, Villejuif, France., Dessen P; Plateforme de Bioinformatique, UMS AMMICA, Gustave Roussy, 39 Rue Camille Desmoulins, F-94805, Villejuif, France., Beq S; HiFiBiO Therapeutics, Pépinière Paris Santé Cochin, 29 Rue du Faubourg Saint-Jacques, F-75014, Paris, France., David M; HiFiBiO Therapeutics, Pépinière Paris Santé Cochin, 29 Rue du Faubourg Saint-Jacques, F-75014, Paris, France., Busson P; CNRS, UMR 9018, Gustave Roussy and Université Paris-Saclay, 39 Rue Camille Desmoulins, F-94805, Villejuif, France. Pierre.Busson@gustaveroussy.fr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Mar 04; Vol. 11 (1), pp. 5227. Date of Electronic Publication: 2021 Mar 04.
DOI: 10.1038/s41598-021-84270-1
Abstrakt: Mechanisms of tumor immune escape are quite diverse and require specific approaches for their exploration in syngeneic tumor models. In several human malignancies, galectin-9 (gal-9) is suspected to contribute to the immune escape. However, in contrast with what has been done for the infiltrating cells, the contribution of gal-9 produced by malignant cells has never been demonstrated in an animal model. Therefore, we derived isogenic clones-either positive or negative for gal-9-from the MB49 murine bladder carcinoma cell line. A progressive and consistent reduction of tumor growth was observed when gal-9-KO cells were subjected to serial transplantations into syngeneic mice. In contrast, tumor growth was unaffected during parallel serial transplantations into nude mice, thus linking tumor inhibition to the enhancement of the immune response against gal-9-KO tumors. This stronger immune response was at least in part explained by changing patterns of response to interferon-γ. One consistent change was a more abundant production of CXCL10, a major inflammatory factor whose production is often induced by interferon-γ. Overall, these observations demonstrate for the first time that serial transplantation into syngeneic mice can be a valuable experimental approach for the exploration of novel mechanisms of tumor immune escape.
Databáze: MEDLINE
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