High-throughput Flow Cytometry Assay to Investigate TDP43 Splicing Function.
| Autor: | Schmidt HB; Department of Biochemistry, Stanford University School of Medicine, Stanford, USA., Rohatgi R; Department of Biochemistry, Stanford University School of Medicine, Stanford, USA.; Department of Medicine, Stanford University School of Medicine, Stanford, USA.; Stanford Cancer Institute, Stanford University School of Medicine, Stanford, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Bio-protocol [Bio Protoc] 2020 Apr 20; Vol. 10 (8), pp. e3594. Date of Electronic Publication: 2020 Apr 20 (Print Publication: 2020). |
| DOI: | 10.21769/BioProtoc.3594 |
| Abstrakt: | Mutations in RNA-binding proteins (RBPs) such as TDP43 are associated with transcriptome-wide splicing defects and cause severe neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The impact of RBP mutations on splicing function is routinely studied using PCR-based bulk measurements. However, the qualitative and low-throughput nature of this assay make quantitative and systematic analyses, as well as screening approaches, difficult to implement. To overcome this hurdle, we have developed a quantitative, high-throughput flow cytometry assay to investigate TDP43 splicing function on a single-cell level. Competing Interests: Competing interestsThe authors declare no competing interests. (Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.) |
| Databáze: | MEDLINE |
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