Maternal Immune Activation with the Viral Mimetic Poly:IC in Pregnant Rats.
| Autor: | Sandini TM; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Canada., Greba Q; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Canada., Lins BR; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Canada., Howland JG; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Canada. |
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| Jazyk: | angličtina |
| Zdroj: | Bio-protocol [Bio Protoc] 2020 Nov 20; Vol. 10 (22), pp. e3817. Date of Electronic Publication: 2020 Nov 20 (Print Publication: 2020). |
| DOI: | 10.21769/BioProtoc.3817 |
| Abstrakt: | Maternal immune activation (MIA) is increasingly well appreciated as an environmental risk factor for some psychiatric disorders. Administration of proinflammatory compounds such as the synthetic double-stranded RNA molecule polyinosinic-polycytidylic acid (polyI:C) to pregnant rodents results in the release of proinflammatory cytokines in the maternal circulation. Various behavioural and brain changes are produced in the offspring that are associated with psychiatric disorders such as autism and schizophrenia. This protocol describes the steps necessary for inducing MIA in pregnant rat dams, which will allow for investigations into the mechanisms in the dam and offspring that mediate the long-term effects of exposure to inflammation while in utero. Increasing our understanding of these mechanisms may provide new insights for the diagnosis, treatment, and prevention of psychiatric disorders. This protocol has been developed and improved over the years by various researchers in Dr. Howland's laboratory at the University of Saskatchewan. Competing Interests: Competing interestsResearch in the Howland laboratory is funded by peer reviewed research grants from government agencies and charities. The authors have no financial or other competing interests to declare. (Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.) |
| Databáze: | MEDLINE |
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