Determination of Microtubule Lattice Parameters from Cryo-electron Microscope Images Using TubuleJ.
| Autor: | Ku S; Univ Rennes, CNRS, IGDR (Institut de genetique et developpement de Rennes) - UMR 6290, F-35000 Rennes, France., Messaoudi C; Institut Curie, PSL Research University, CNRS UMS 2016, F-91401 Orsay, France.; Université Paris-Saclay, INSERM US43, F-91401 Orsay, France., Guyomar C; Univ Rennes, CNRS, IGDR (Institut de genetique et developpement de Rennes) - UMR 6290, F-35000 Rennes, France., Kervrann C; Inria-Centre de Rennes Bretagne Atlantique, Campus Universitaire de Beaulieu, 35042 Rennes, France., Chrétien D; Univ Rennes, CNRS, IGDR (Institut de genetique et developpement de Rennes) - UMR 6290, F-35000 Rennes, France. |
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| Jazyk: | angličtina |
| Zdroj: | Bio-protocol [Bio Protoc] 2020 Nov 05; Vol. 10 (21), pp. e3814. Date of Electronic Publication: 2020 Nov 05 (Print Publication: 2020). |
| DOI: | 10.21769/BioProtoc.3814 |
| Abstrakt: | The α-β tubulin heterodimer undergoes subtle conformational changes during microtubule assembly. These can be modulated by external factors, whose effects on microtubule structure can be characterized on 2D views obtained by cryo-electron microscopy. Analysis of microtubule images is facilitated if they are straight enough to interpret and filter their image Fourier transform, which provide useful information concerning the arrangement of tubulin molecules inside the microtubule lattice. Here, we describe the use of the TubuleJ software to straighten microtubules and determine their lattice parameters. Basic 3D reconstructions can be performed to evaluate the relevance of these parameters. This approach can be used to analyze the effects of nucleotide analogues, drugs or MAPs on microtubule structure, or to select microtubule images prior to high-resolution 3D reconstructions. Competing Interests: Competing interestsTubuleJ was deposited to the Agency for the Protection of programs (APP) under the code number IDDN.FR.001.240023.000.S.P.2011.000.21000. (Copyright © The Authors; exclusive licensee Bio-protocol LLC.) |
| Databáze: | MEDLINE |
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