Quantitative conversion of biomass in giant DNA virus infection.

Autor: Kördel M; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, 106 91, Stockholm, Sweden. mikael.kordel@biox.kth.se., Svenda M; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, 106 91, Stockholm, Sweden.; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), 751 24, Uppsala, Sweden., Reddy HKN; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), 751 24, Uppsala, Sweden., Fogelqvist E; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, 106 91, Stockholm, Sweden., Arsana KGY; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, 106 91, Stockholm, Sweden., Hamawandi B; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, 106 91, Stockholm, Sweden., Toprak MS; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, 106 91, Stockholm, Sweden., Hertz HM; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, 106 91, Stockholm, Sweden., Sellberg JA; Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, 106 91, Stockholm, Sweden. jonassel@kth.se.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Mar 03; Vol. 11 (1), pp. 5025. Date of Electronic Publication: 2021 Mar 03.
DOI: 10.1038/s41598-021-83547-9
Abstrakt: Bioconversion of organic materials is the foundation of many applications in chemical engineering, microbiology and biochemistry. Herein, we introduce a new methodology to quantitatively determine conversion of biomass in viral infections while simultaneously imaging morphological changes of the host cell. As proof of concept, the viral replication of an unidentified giant DNA virus and the cellular response of an amoebal host are studied using soft X-ray microscopy, titration dilution measurements and thermal gravimetric analysis. We find that virions produced inside the cell are visible from 18 h post infection and their numbers increase gradually to a burst size of 280-660 virions. Due to the large size of the virion and its strong X-ray absorption contrast, we estimate that the burst size corresponds to a conversion of 6-12% of carbonaceous biomass from amoebal host to virus. The occurrence of virion production correlates with the appearance of a possible viral factory and morphological changes in the phagosomes and contractile vacuole complex of the amoeba, whereas the nucleus and nucleolus appear unaffected throughout most of the replication cycle.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje