Mass spectrometry and the cellular surfaceome.

Autor: Pauwels J; VIB Center for Medical Biotechnology, VIB, Ghent, Belgium.; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium., Fijałkowska D; VIB Center for Medical Biotechnology, VIB, Ghent, Belgium., Eyckerman S; VIB Center for Medical Biotechnology, VIB, Ghent, Belgium.; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium., Gevaert K; VIB Center for Medical Biotechnology, VIB, Ghent, Belgium.; Department of Biomolecular Medicine, Ghent University, Ghent, Belgium.
Jazyk: angličtina
Zdroj: Mass spectrometry reviews [Mass Spectrom Rev] 2022 Sep; Vol. 41 (5), pp. 804-841. Date of Electronic Publication: 2021 Mar 02.
DOI: 10.1002/mas.21690
Abstrakt: The collection of exposed plasma membrane proteins, collectively termed the surfaceome, is involved in multiple vital cellular processes, such as the communication of cells with their surroundings and the regulation of transport across the lipid bilayer. The surfaceome also plays key roles in the immune system by recognizing and presenting antigens, with its possible malfunctioning linked to disease. Surface proteins have long been explored as potential cell markers, disease biomarkers, and therapeutic drug targets. Despite its importance, a detailed study of the surfaceome continues to pose major challenges for mass spectrometry-driven proteomics due to the inherent biophysical characteristics of surface proteins. Their inefficient extraction from hydrophobic membranes to an aqueous medium and their lower abundance compared to intracellular proteins hamper the analysis of surface proteins, which are therefore usually underrepresented in proteomic datasets. To tackle such problems, several innovative analytical methodologies have been developed. This review aims at providing an extensive overview of the different methods for surfaceome analysis, with respective considerations for downstream mass spectrometry-based proteomics.
(© 2021 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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