pNP Transgenic RNAi System Manual in Drosophila .
| Autor: | Wang F; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China., Qiao HH; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China., Xu RG; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China., Sun J; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China., Zhu R; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China., Mao D; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China., Ni JQ; Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.; Tsingdao Advanced Research Institute, Tongji University, Qingdao 266000, China. |
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| Jazyk: | angličtina |
| Zdroj: | Bio-protocol [Bio Protoc] 2019 Feb 05; Vol. 9 (3), pp. e3158. Date of Electronic Publication: 2019 Feb 05 (Print Publication: 2019). |
| DOI: | 10.21769/BioProtoc.3158 |
| Abstrakt: | Much of our knowledge about the mechanisms underlying biological processes relies on genetic approaches, whereby gene activity is reduced and the phenotypic consequences of perturbation are analyzed in detail. For functional genomic studies, a specific, systematic, and cost-effective manner is critical. Transgenic RNAi system is the top priority choice to study gene functions due to its simple and practical characteristics in Drosophila . We established a novel system that works well in both soma and germ cells which is efficient and specific. With this system, we can precisely and efficiently modulate highly expressed genes, and simultaneously knock down multiple genes in one step. In this study, we provide a detailed protocol of the pNP system, which replaces other transgenic systems, and expect it can provide some help to researchers who are using this system. Competing Interests: Competing interestsThe authors declare no competing financial interests. (Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.) |
| Databáze: | MEDLINE |
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