Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal.

Autor:	Fazakerley DJ; Charles Perkins Centre, School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales 2006, Australia., Fritzen AM; Charles Perkins Centre, School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales 2006, Australia.; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Copenhagen 2200, Denmark., Nelson ME; Charles Perkins Centre, School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales 2006, Australia., Thorius IH; Charles Perkins Centre, School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales 2006, Australia.; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Copenhagen 2200, Denmark., Cooke KC; Charles Perkins Centre, School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales 2006, Australia., Humphrey SJ; Charles Perkins Centre, School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales 2006, Australia., Cooney GJ; Charles Perkins Centre, Sydney Medical School, University of Sydney, Camperdown, New South Wales 2006, Australia., James DE; Charles Perkins Centre, School of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales 2006, Australia.; Charles Perkins Centre, Sydney Medical School, University of Sydney, Camperdown, New South Wales 2006, Australia.
Jazyk:	angličtina
Zdroj:	Bio-protocol [Bio Protoc] 2019 Jan 20; Vol. 9 (2), pp. e3146. Date of Electronic Publication: 2019 Jan 20 (Print Publication: 2019).
DOI:	10.21769/BioProtoc.3146
Abstrakt:	Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic clamps permit assessment of insulin responses in specific tissues and allow the study of the progression and causes of insulin resistance. Here we detail a protocol for assessing insulin action in adipose and muscle tissues in anesthetized mice administered with insulin intravenously. Competing Interests: Competing interestsThe authors have no competing interests to declare. (Copyright © The Authors; exclusive licensee Bio-protocol LLC.)
Databáze:	MEDLINE
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