A Mycobacterial Systems Resource for the Research Community.

Autor:	Judd JA; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Canestrari J; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Clark R; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Joseph A; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Lapierre P; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Lasek-Nesselquist E; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Mir M; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Palumbo M; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Smith C; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Stone M; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Upadhyay A; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Wirth SE; Wadsworth Center, New York State Department of Health, Albany, New York, USA., Dedrick RM; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Meier CG; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Russell DA; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Dills A; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA., Dove E; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA., Kester J; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA., Wolf ID; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA., Zhu J; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA., Rubin ER; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA., Fortune S; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA., Hatfull GF; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Gray TA; Wadsworth Center, New York State Department of Health, Albany, New York, USA todd.gray@health.ny.gov joseph.wade@health.ny.gov keith.derbyshire@health.ny.gov.; Department of Biomedical Sciences, University at Albany, Albany, New York, USA., Wade JT; Wadsworth Center, New York State Department of Health, Albany, New York, USA todd.gray@health.ny.gov joseph.wade@health.ny.gov keith.derbyshire@health.ny.gov.; Department of Biomedical Sciences, University at Albany, Albany, New York, USA., Derbyshire KM; Wadsworth Center, New York State Department of Health, Albany, New York, USA todd.gray@health.ny.gov joseph.wade@health.ny.gov keith.derbyshire@health.ny.gov.; Department of Biomedical Sciences, University at Albany, Albany, New York, USA.
Jazyk:	angličtina
Zdroj:	MBio [mBio] 2021 Mar 02; Vol. 12 (2). Date of Electronic Publication: 2021 Mar 02.
DOI:	10.1128/mBio.02401-20
Abstrakt:	Functional characterization of bacterial proteins lags far behind the identification of new protein families. This is especially true for bacterial species that are more difficult to grow and genetically manipulate than model systems such as Escherichia coli and Bacillus subtilis To facilitate functional characterization of mycobacterial proteins, we have established a Mycobacterial Systems Resource (MSR) using the model organism Mycobacterium smegmatis This resource focuses specifically on 1,153 highly conserved core genes that are common to many mycobacterial species, including Mycobacterium tuberculosis , in order to provide the most relevant information and resources for the mycobacterial research community. The MSR includes both biological and bioinformatic resources. The biological resource includes (i) an expression plasmid library of 1,116 genes fused to a fluorescent protein for determining protein localization; (ii) a library of 569 precise deletions of nonessential genes; and (iii) a set of 843 CRISPR-interference (CRISPRi) plasmids specifically targeted to silence expression of essential core genes and genes for which a precise deletion was not obtained. The bioinformatic resource includes information about individual genes and a detailed assessment of protein localization. We anticipate that integration of these initial functional analyses and the availability of the biological resource will facilitate studies of these core proteins in many Mycobacterium species, including the less experimentally tractable pathogens M. abscessus , M. avium , M. kansasii , M. leprae , M. marinum , M. tuberculosis , and M. ulcerans IMPORTANCE Diseases caused by mycobacterial species result in millions of deaths per year globally, and present a substantial health and economic burden, especially in immunocompromised patients. Difficulties inherent in working with mycobacterial pathogens have hampered the development and application of high-throughput genetics that can inform genome annotations and subsequent functional assays. To facilitate mycobacterial research, we have created a biological and bioinformatic resource (https://msrdb.org/) using Mycobacterium smegmatis as a model organism. The resource focuses specifically on 1,153 proteins that are highly conserved across the mycobacterial genus and, therefore, likely perform conserved mycobacterial core functions. Thus, functional insights from the MSR will apply to all mycobacterial species. We believe that the availability of this mycobacterial systems resource will accelerate research throughout the mycobacterial research community. (Copyright © 2021 Judd et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu