Low-Dose Coconut Oil Supplementation Induces Hypothalamic Inflammation, Behavioral Dysfunction, and Metabolic Damage in Healthy Mice.

Autor: Veras ACC; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, Limeira, Brazil., Santos TD; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, Limeira, Brazil., Martins ICA; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, Limeira, Brazil., de Souza CM; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, Limeira, Brazil., Amaral CL; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, Limeira, Brazil., Franco BDS; Laboratory of Sleep and Exercise, School of Applied Sciences, University of Campinas, Limeira, Brazil., Holanda ASS; Laboratory of Sleep and Exercise, School of Applied Sciences, University of Campinas, Limeira, Brazil., Esteves AM; Laboratory of Sleep and Exercise, School of Applied Sciences, University of Campinas, Limeira, Brazil., Milanski M; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, Limeira, Brazil.; Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil., Torsoni AS; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, Limeira, Brazil.; Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil., Ignacio-Souza LM; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, Limeira, Brazil.; Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil., Torsoni MA; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, Limeira, Brazil.; Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil.
Jazyk: angličtina
Zdroj: Molecular nutrition & food research [Mol Nutr Food Res] 2021 May; Vol. 65 (10), pp. e2000943. Date of Electronic Publication: 2021 Apr 17.
DOI: 10.1002/mnfr.202000943
Abstrakt: Scope: Coconut oil (CO) diets remain controversial due to the possible association with metabolic disorder and obesity. This study investigates the metabolic effects of a low amount of CO supplementation.
Methods and Results: Swiss male mice are assigned to be supplemented orally during 8 weeks with 300 µL of water for the control group (CV), 100 or 300 µL of CO (CO100 and CO300) and 100 or 300 µL of soybean oil (SO; SO100 and SO300). CO led to anxious behavior, increase in body weight gain, and adiposity. In the hypothalamus, CO and SO increase cytokines expression and pJNK, pNFKB, and TLR4 levels. Nevertheless, the adipose tissue presented increases macrophage infiltration, TNF-α and IL-6 after CO and SO consumption. IL-1B and CCL2 expression, pJNK and pNFKB levels increase only in CO300. In the hepatic tissue, CO increases TNF-α and chemokines expression. Neuronal cell line (mHypoA-2/29) exposed to serum from CO and SO mice shows increased NFKB migration to the nucleus, TNF-α, and NFKBia expression, but are prevented by inhibitor of TLR4 (TAK-242).
Conclusions: These results show that a low-dose CO changes the behavioral pattern, induces inflammatory pathway activation, TLR4 expression in healthy mice, and stimulates the pro-inflammatory response through a TLR4-mediated mechanism.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
Externí odkaz: