Anti-inflammatory Properties of Ethanolic Extract and 2″-O-β-D-Glucopyranosyl-vitexin Obtained from Alternanthera tenella Colla Whole Plant.

Autor: Kassuya RM; College of Health Science, Federal University of Grande Dourados, Dourados, MS, Brazil., Dos Santos E; College of Health Science, Federal University of Grande Dourados, Dourados, MS, Brazil., Bosso FH; Institute of Biology, Department of Plant Biology, PPG BTPB, PPG BV and PPG BCE, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Pedroso TF; Institute of Biology, Department of Plant Biology, PPG BTPB, PPG BV and PPG BCE, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Marinho JVN; Institute of Biology, Department of Plant Biology, PPG BTPB, PPG BV and PPG BCE, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Salvador MJ; Institute of Biology, Department of Plant Biology, PPG BTPB, PPG BV and PPG BCE, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Kassuya CAL; College of Health Science, Federal University of Grande Dourados, Dourados, MS, Brazil. candida2005@gmail.com., Gasparotto Junior A; College of Health Science, Federal University of Grande Dourados, Dourados, MS, Brazil.
Jazyk: angličtina
Zdroj: Inflammation [Inflammation] 2021 Aug; Vol. 44 (4), pp. 1540-1552. Date of Electronic Publication: 2021 Mar 01.
DOI: 10.1007/s10753-021-01438-7
Abstrakt: Six flavonoids were identified and isolated from the ethanolic extract of Alternanthera tenella Colla (Amaranthaceae) whole plant (EEAT) including 2″-O-β-D-glucopyranosyl-vitexin (A19). Flavonoids have anti-inflammatory activity; however, the 2″-O-β-D-glucopyranosyl-vitexin anti-inflammatory property was not totally explored. The aim of the present study was to investigate the anti-inflammatory effects of ethanolic extract from A. tenella whole plant and isolated flavone C-glycoside A19 in models of inflammation. The oral administration (p.o.) of EEAT (30, 100, and 300 mg/kg), A19 (0.1, 1, and 10 mg/kg), and prednisolone (3 mg/kg) were tested against the carrageenan-induced paw edema in Swiss mice. The EEAT (100 mg/kg, p.o.), A19 (1 mg/kg, p.o.), and prednisolone (3 mg/kg, p.o.) were tested in the zymosan-articular inflammation, carrageenan-pleurisy, and complete Freund's adjuvant (CFA) models in Swiss mice. In silico analysis and search for structural relationships between vitexin derivatives flavones present in the EEAT and TNF-α inhibitors were performed. EEAT, A19, and prednisolone significantly inhibited (i) edema, mechanical hyperalgesia in carrageenan-induced paw inflammation; (ii) leukocyte migration and protein extravasation in carrageenan-induced pleurisy; (iii) knee edema, mechanical hyperalgesia, and leukocyte migration in articular inflammation induced by zymosan. Still the CFA induced the increase in myeloperoxidase and N-acetylglucosaminidase activities, EEAT, A19, and prednisolone significantly inhibited these enzymes. The in silico analysis showed that molecules with similar structure to apigenin and vitexin have a potential inhibition on the TNF system. This study confirms the anti-inflammatory properties of EEAT and A19. The C-glycosylated flavone A19 could be responsible for the EEAT anti-edematogenic and anti-hyperalgesic effects and a potential TNF-α inhibitor.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.)
Databáze: MEDLINE
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