Unique Wolbachia strain wMelPlus increases heat stress resistance in Drosophila melanogaster.

Autor: Burdina EV; Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia., Bykov RA; Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia., Menshanov PN; Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.; Novosibirsk State Technical University, Novosibirsk, Russia.; Novosibirsk State University, Novosibirsk, Russia., Ilinsky YY; Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia., Gruntenko NЕ; Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Archives of insect biochemistry and physiology [Arch Insect Biochem Physiol] 2021 Apr; Vol. 106 (4), pp. e21776. Date of Electronic Publication: 2021 Feb 28.
DOI: 10.1002/arch.21776
Abstrakt: Maternally inherited endosymbiotic bacterium Wolbachia infects Drosophila melanogaster populations worldwide. Its genetic diversity includes several closely related genotypes, which can be attributed to two main genotype groups: wMel and wMelCS. Here, we studied eight D. melanogaster lines carrying the nuclear background of wild type interbred Bi90 line and cytoplasmic backgrounds with or without Wolbachia of different origin, each of which belongs to wMelCS genotype group. We analyzed the effect these seven Wolbachia strains had on the heat stress resistance and dopamine metabolism in D. melanogaster females. Survival under heat stress (38°C, 3 h 30 min) was increased only in the line infected with bacteria of the wMelPlus strain. At the same time, the activity of alkaline phosphatase (an enzyme regulating the pool of dopamine precursor tyrosine) was increased under normal conditions in females infected with all strains under study and retained the response to heat stress typical for the uninfected line. Thus, we found the unique Wolbachia strain that provides an increase of the host stress resistance, and demonstrated that the mechanism of this resistance is not dopamine-mediated.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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