Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters ( Crassostrea gasar ).

Autor:	Conceição MVR; Laboratory of Genomics and Bioinformatics, Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.; Laboratory of Biological Engineering, Guamá Science and Technology Park, Belém, Brazil., Costa SS; Laboratory of Genomics and Bioinformatics, Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.; Laboratory of Biological Engineering, Guamá Science and Technology Park, Belém, Brazil., Schaan AP; Laboratory of Medical and Human Genetics, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil., Ribeiro-Dos-Santos ÂKC; Laboratory of Medical and Human Genetics, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil., Silva A; Laboratory of Genomics and Bioinformatics, Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.; Laboratory of Biological Engineering, Guamá Science and Technology Park, Belém, Brazil., das Graças DA; Laboratory of Genomics and Bioinformatics, Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.; Laboratory of Biological Engineering, Guamá Science and Technology Park, Belém, Brazil., Schneider MPC; Laboratory of Genomics and Bioinformatics, Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.; Laboratory of Biological Engineering, Guamá Science and Technology Park, Belém, Brazil., Baraúna RA; Laboratory of Genomics and Bioinformatics, Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.; Laboratory of Biological Engineering, Guamá Science and Technology Park, Belém, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in genetics [Front Genet] 2021 Feb 12; Vol. 11, pp. 602608. Date of Electronic Publication: 2021 Feb 12 (Print Publication: 2020).
DOI:	10.3389/fgene.2020.602608
Abstrakt:	The mangrove oysters ( Crassostrea gasar ) are molluscs native to the Amazonia region and their exploration and farming has increased considerably in recent years. These animals are farmed on beds built in the rivers of the Amazonia estuaries and, therefore, the composition of their microbiome should be directly influenced by environmental conditions. Our work aimed to evaluate the changes in bacterial composition of oyster's microbiota at two different seasons (rainy and dry). For this purpose, we amplified and sequenced the V3-V4 regions of the 16S rRNA gene. Sequencing was performed on the Illumina MiSeq platform. According to the rarefaction curve, the sampling effort was sufficient to describe the bacterial diversity in the samples. Alpha-diversity indexes showed that the bacterial microbiota of oysters is richer during the rainy season. This richness is possibly associated with the diversity at lower taxonomic levels, since the relative abundance of bacterial phyla in the two seasons remained relatively constant. The main phyla found include Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria. Similar results were found for the species Crassostrea gigas, Crassostrea sikamea , and Crassostrea corteziensis . Beta-diversity analysis showed that the bacterial composition of oyster's gut microbiota was quite different in the two seasons. Our data demonstrate the close relationship between the environment and the microbiome of these molluscs, reinforcing the need for conservation and sustainable management of estuaries in the Amazonia. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Conceição, Costa, Schaan, Ribeiro-dos-Santos, Silva, das Graças, Schneider and Baraúna.)
Databáze:	MEDLINE
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