Ras2 is important for growth and pathogenicity in Fusarium circinatum.

Autor: Phasha MM; Departments of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa. Electronic address: mmatshepho.phasha@fabi.up.ac.za., Wingfield MJ; Departments of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa. Electronic address: mike.wingfield@fabi.up.ac.za., Wingfield BD; Departments of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa. Electronic address: brenda.wingfield@fabi.up.ac.za., Coetzee MPA; Departments of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa. Electronic address: martin.coetzee@fabi.up.ac.za., Hallen-Adams H; Food Science and Technology Department, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, USA. Electronic address: hhallen-adams2@unl.edu., Fru F; Departments of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa. Electronic address: felix.fru@fabi.up.ac.za., Swalarsk-Parry BS; Departments of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa. Electronic address: benny.swalarsk@fabi.up.ac.za., Yilmaz N; Departments of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa. Electronic address: neriman.yilmazvisagie@fabi.up.ac.za., Duong TA; Departments of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa. Electronic address: tuan.duong@fabi.up.ac.za., Steenkamp ET; Departments of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa. Electronic address: emma.steenkamp@fabi.up.ac.za.
Jazyk: angličtina
Zdroj: Fungal genetics and biology : FG & B [Fungal Genet Biol] 2021 May; Vol. 150, pp. 103541. Date of Electronic Publication: 2021 Feb 24.
DOI: 10.1016/j.fgb.2021.103541
Abstrakt: In this study, we investigated to possible role of Ras2 in Fusarium circinatum- a fungus that causes pine pitch canker disease on many different pine species and has a wide geographic distribution. This protein is encoded by the RAS2 gene and has been shown to control growth and pathogenicity in a number of fungi in a mitogen-activated protein kinase- and/or cyclic adenosyl monophosphate pathway-dependent manner. The aim was therefore to characterize the phenotypes of RAS2 gene knockout and complementation mutants of F. circinatum. These mutants were generated by transforming protoplasts of the fungus with suitable split-marker constructs. The mutant strains, together with the wild type strain, were used in growth studies as well as pathogenicity assays on Pinus patula seedlings. Results showed that the knockout mutant strain produced significantly smaller lesions compared to the complementation mutant and wild type strains. Growth studies also showed significantly smaller colonies and delayed conidial germination in the knockout mutant strain compared to the complement mutant and wild type strains. Interestingly, the knockout mutant strain produced more macroconidia than the wild type strain. Collectively, these results showed that Ras2 plays an important role in both growth and pathogenicity of F. circinatum. Future studies will seek to determine the pathway(s) through which Ras2 controls these traits in F. circinatum.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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