In vitro Selection of Probiotics for Microbiota Modulation in Normal-Weight and Severely Obese Individuals: Focus on Gas Production and Interaction With Intestinal Epithelial Cells.

Autor:	Nogacka AM; Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Spain.; Diet, Microbiota and Health Group, Institute of Health Research of the Principality of Asturias (ISPA), Oviedo, Spain., de Los Reyes-Gavilán CG; Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Spain.; Diet, Microbiota and Health Group, Institute of Health Research of the Principality of Asturias (ISPA), Oviedo, Spain., Arboleya S; Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Spain.; Diet, Microbiota and Health Group, Institute of Health Research of the Principality of Asturias (ISPA), Oviedo, Spain., Ruas-Madiedo P; Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Spain.; Functionality and Ecology of Beneficial Microorganisms, Institute of Health Research of the Principality of Asturias (ISPA), Oviedo, Spain., Martínez-Faedo C; Endocrinology and Nutrition Service, Central University Hospital of Asturias (HUCA), Oviedo, Spain.; Endocrinology, Nutrition, Diabetes and Obesity Group, Institute of Health Research of the Principality of Asturias (ISPA), Oviedo, Spain., Suarez A; Diet, Microbiota and Health Group, Institute of Health Research of the Principality of Asturias (ISPA), Oviedo, Spain.; Digestive Service, Central University Hospital of Asturias (HUCA), Oviedo, Spain., He F; Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama, Japan., Harata G; Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama, Japan., Endo A; Department of Food and Cosmetic Science, Tokyo University of Agriculture, Abashiri, Japan., Salazar N; Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Spain.; Diet, Microbiota and Health Group, Institute of Health Research of the Principality of Asturias (ISPA), Oviedo, Spain., Gueimonde M; Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias, Consejo Superior de Investigaciones Científicas (IPLA-CSIC), Villaviciosa, Spain.; Diet, Microbiota and Health Group, Institute of Health Research of the Principality of Asturias (ISPA), Oviedo, Spain.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2021 Feb 09; Vol. 12, pp. 630572. Date of Electronic Publication: 2021 Feb 09 (Print Publication: 2021).
DOI:	10.3389/fmicb.2021.630572
Abstrakt:	The intestinal microbiota plays important roles in the maintenance of health. Strategies aiming at its modulation, such as probiotics, have received a deal of attention. Several strains have been studied in different in vitro models; however, the correlation of results obtained with the in vivo data has been limited. This questions the usefulness of such in vitro selection models, traditionally relying on over-simplified tests, not considering the influence of the accompanying microbiota or focusing on microbiota composition without considering functional traits. Here we assess the potential of six Bifidobacterium , Lactobacillus and Lacticaseibacillus strains in an in vitro model to determine their impact on the microbiota not just in terms of composition but also of functionality. Moreover, we compared the responses obtained in two different population groups: normal-weight and severely obese subjects. Fecal cultures were conducted to evaluate the impact of the strains on specific intestinal microbial groups, on the production of short-chain fatty acids, and on two functional responses: the production of gas and the interaction with human intestinal epithelial cells. The response to the different probiotics differed between both human groups. The addition of the probiotic strains did not induce major changes on the microbiota composition, with significant increases detected almost exclusively for the species added. Higher levels of gas production were observed in cultures from normal-weight subjects than in the obese population, with some strains being able to significantly reduce gas production in the latter group. Moreover, in obese subjects all the Bifidobacterium strains tested and Lacticaseibacillus rhamnosus GG were able to modify the response of the intestinal cells, restoring values similar to those obtained with the microbiotas of normal-weight subjects. Our results underline the need for the screening and selection of probiotics in a target-population specific manner by using appropriate in vitro models before enrolling in clinical intervention trials. Competing Interests: FH and GH were employed by the company Takanashi Milk Products Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be constructed as potential conflict of interest. The authors declare that this study received funding from Takanashi Milk products. The funder had the following involvement with the study: study design. (Copyright © 2021 Nogacka, de los Reyes-Gavilán, Arboleya, Ruas-Madiedo, Martínez-Faedo, Suarez, He, Harata, Endo, Salazar and Gueimonde.)
Databáze:	MEDLINE
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