Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire.

Autor: Penna B; Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Silva MB; Laboratório de Sanidade Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil., Soares AER; Laboratório Nacional de Computação Científica, Petrópolis, Rio de Janeiro, Brazil., Vasconcelos ATR; Laboratório Nacional de Computação Científica, Petrópolis, Rio de Janeiro, Brazil., Ramundo MS; Department of Medical Microbiology, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Ferreira FA; Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil., Silva-Carvalho MC; Department of Medical Microbiology, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Sousa VS; Department of Medical Microbiology, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Rabello RF; Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Bandeira PT; Department of Medical Microbiology, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.; Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Souza VS; Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Planet PJ; Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Vieira-da-Motta O; Laboratório de Sanidade Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil., Botelho AMN; Instituto Biomédico, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. abotelho@id.uff.br.; Department of Medical Microbiology, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. abotelho@id.uff.br., Figueiredo AMS; Department of Medical Microbiology, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. agnes@micro.ufrj.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Feb 25; Vol. 11 (1), pp. 4724. Date of Electronic Publication: 2021 Feb 25.
DOI: 10.1038/s41598-021-83993-5
Abstrakt: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen associated with a wide variety of infections in humans. The ability of MRSA to infect companion animals has gained increasing attention in the scientific literature. In this study, 334 dogs were screened for MRSA in two cities located in Rio de Janeiro State. The prevalence of MRSA in dogs was 2.7%. Genotyping revealed isolates from sequence types (ST) 1, 5, 30, and 239 either colonizing or infecting dogs. The genome of the canine ST5 MRSA (strain SA112) was compared with ST5 MRSA from humans-the main lineage found in Rio de Janeiro hospitals-to gain insights in the origin of this dog isolate. Phylogenetic analysis situated the canine genome and human strain CR14-035 in the same clade. Comparative genomics revealed similar virulence profiles for SA112 and CR14-035. Both genomes carry S. aureus genomic islands νSAα, νSAβ, and νSAγ. The virulence potential of the canine and human strains was similar in a Caenorhabditis elegans model. Together, these results suggest a potential of canine MRSA to infect humans and vice versa. The circulation in community settings of a MRSA lineage commonly found in hospitals is an additional challenge for public health surveillance authorities.
Databáze: MEDLINE
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