PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula.

Autor:	Nguyen NNT; Department of Plant Molecular Biology, Biophore Building, University of Lausanne, Lausanne 1015, Switzerland., Clua J; Department of Plant Molecular Biology, Biophore Building, University of Lausanne, Lausanne 1015, Switzerland., Vetal PV; Department of Plant Molecular Biology, Biophore Building, University of Lausanne, Lausanne 1015, Switzerland., Vuarambon DJ; Department of Plant Molecular Biology, Biophore Building, University of Lausanne, Lausanne 1015, Switzerland., De Bellis D; Department of Plant Molecular Biology, Biophore Building, University of Lausanne, Lausanne 1015, Switzerland.; Electron Microscopy Facility, Biophore Building, University of Lausanne, Lausanne 1015, Switzerland., Pervent M; Laboratoire des Symbioses Tropicales et Méditerranéennes UMR 1342 INRAE-IRD-CIRAD-UM-Montpellier SupAgro, Montpellier, France., Lepetit M; Laboratoire des Symbioses Tropicales et Méditerranéennes UMR 1342 INRAE-IRD-CIRAD-UM-Montpellier SupAgro, Montpellier, France., Udvardi M; The Noble Research Institute, 2510 Sam Noble Parkway, Ardmore, OK, USA., Valentine AJ; Botany & Zoology Department, University of Stellenbosch, Matieland 7602, South Africa., Poirier Y; Department of Plant Molecular Biology, Biophore Building, University of Lausanne, Lausanne 1015, Switzerland.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2021 Feb 25; Vol. 185 (1), pp. 196-209.
DOI:	10.1093/plphys/kiaa016
Abstrakt:	Legumes play an important role in the soil nitrogen availability via symbiotic nitrogen fixation (SNF). Phosphate (Pi) deficiency severely impacts SNF because of the high Pi requirement of symbiosis. Whereas PHT1 transporters are involved in Pi uptake into nodules, it is unknown how Pi is transferred from the plant infected cells to nitrogen-fixing bacteroids. We hypothesized that Medicago truncatula genes homologous to Arabidopsis PHO1, encoding a vascular apoplastic Pi exporter, are involved in Pi transfer to bacteroids. Among the seven MtPHO1 genes present in M. truncatula, we found that two genes, namely MtPHO1.1 and MtPHO1.2, were broadly expressed across the various nodule zones in addition to the root vascular system. Expressions of MtPHO1.1 and MtPHO1.2 in Nicotiana benthamiana mediated specific Pi export. Plants with nodule-specific downregulation of both MtPHO1.1 and MtPHO1.2 were generated by RNA interference (RNAi) to examine their roles in nodule Pi homeostasis. Nodules of RNAi plants had lower Pi content and a three-fold reduction in SNF, resulting in reduced shoot growth. Whereas the rate of 33Pi uptake into nodules of RNAi plants was similar to control, transfer of 33Pi from nodule cells into bacteroids was reduced and bacteroids activated their Pi-deficiency response. Our results implicate plant MtPHO1 genes in bacteroid Pi homeostasis and SNF via the transfer of Pi from nodule infected cells to bacteroids. (© The Author(s) 2020. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu