Whole genome genetic variation and linkage disequilibrium in a diverse collection of Listeria monocytogenes isolates.

Autor: Louha S; Institute of Bioinformatics, University of Georgia, Athens, GA, United States of America., Meinersmann RJ; USDA Agricultural Research Service, U.S. National Poultry Research Center, Athens, GA, United States of America., Glenn TC; Institute of Bioinformatics, University of Georgia, Athens, GA, United States of America.; Department of Environmental Health Science, University of Georgia, Athens, GA, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Feb 25; Vol. 16 (2), pp. e0242297. Date of Electronic Publication: 2021 Feb 25 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0242297
Abstrakt: We performed whole-genome multi-locus sequence typing for 2554 genes in a large and heterogenous panel of 180 Listeria monocytogenes strains having diverse geographical and temporal origins. The subtyping data was used for characterizing genetic variation and evaluating patterns of linkage disequilibrium in the pan-genome of L. monocytogenes. Our analysis revealed the presence of strong linkage disequilibrium in L. monocytogenes, with ~99% of genes showing significant non-random associations with a large majority of other genes in the genome. Twenty-seven loci having lower levels of association with other genes were considered to be potential "hot spots" for horizontal gene transfer (i.e., recombination via conjugation, transduction, and/or transformation). The patterns of linkage disequilibrium in L. monocytogenes suggest limited exchange of foreign genetic material in the genome and can be used as a tool for identifying new recombinant strains. This can help understand processes contributing to the diversification and evolution of this pathogenic bacteria, thereby facilitating development of effective control measures.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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