Regeneration of pulp-dentin complex using human stem cells of the apical papilla: in vivo interaction with two bioactive materials.

Autor: Sequeira DB; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal., Oliveira AR; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; MIT Portugal PhD Program, Universidade Nova de Lisboa, Lisbon, Portugal., Seabra CM; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; Institute for Interdisciplinary Research, University of Coimbra, Coimbra, Portugal., Palma PJ; Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal., Ramos C; Coimbra Institute for Clinical and Biomedical Research (iCBR) - CIBB, University of Coimbra, Coimbra, Portugal.; Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal., Figueiredo MH; Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal., Santos AC; Coimbra Institute for Clinical and Biomedical Research (iCBR) - CIBB, University of Coimbra, Coimbra, Portugal.; Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal., Cardoso AL; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; Institute for Interdisciplinary Research, University of Coimbra, Coimbra, Portugal., Peça J; CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.; Department of Life Sciences, University of Coimbra, Coimbra, Portugal., Santos JM; Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal. jsantos@fmed.uc.pt.; Institute for Interdisciplinary Research, University of Coimbra, Coimbra, Portugal. jsantos@fmed.uc.pt.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2021 Sep; Vol. 25 (9), pp. 5317-5329. Date of Electronic Publication: 2021 Feb 25.
DOI: 10.1007/s00784-021-03840-9
Abstrakt: Objectives: To compare the regenerative properties of human stem cells of the apical papilla (SCAPs) embedded in a platelet-rich plasma (PRP) scaffold, when implanted in vivo using an organotypic model composed of human root segments, with or without the presence of the bioactive cements - ProRoot MTA or Biodentine.
Material and Methods: SCAPs were isolated from third molars with incomplete rhizogenesis and expanded and characterized in vitro using stem cell and surface markers. The pluripotency of these cells was also assessed using adipogenic, chondrogenic, and osteogenic differentiation protocols. SCAPs together with a scaffold of PRP were added to the root segment lumen and the organotypic model implanted on the dorsal region of immunodeficient rats for a period of 4 months.
Results: Presence of SCAPs induced de novo formation of dentin-like and pulp-like tissue. A barrier of either ProRoot MTA or Biodentine did not significantly affect the fraction of sections from roots segments observed to contain deposition of hard material (P > 0.05). However, the area of newly deposited dentin was significantly greater in segments containing a barrier of Biodentine compared with ProRoot MTA (P < 0.001).
Conclusions and Clinical Relevance: SCAPs offer a viable alternative to other dental stem cells (DSCs) in their regenerative properties when enclosed in the microenvironment of human tooth roots. The present study also suggests that the presence of bioactive materials does not hinder or impede the formation of new hard tissues, but the presence of Biodentine may promote greater mineralized tissue deposition.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
Databáze: MEDLINE
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