Mutations introduced in susceptibility genes through CRISPR/Cas9 genome editing confer increased late blight resistance in potatoes.

Autor: Kieu NP; Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden., Lenman M; Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden., Wang ES; Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden., Petersen BL; Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsen's vej 40, 1871, Frederiksberg C, Denmark., Andreasson E; Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden. erik.andreasson@slu.se.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Feb 24; Vol. 11 (1), pp. 4487. Date of Electronic Publication: 2021 Feb 24.
DOI: 10.1038/s41598-021-83972-w
Abstrakt: The use of pathogen-resistant cultivars is expected to increase yield and decrease fungicide use in agriculture. However, in potato breeding, increased resistance obtained via resistance genes (R-genes) is hampered because R-gene(s) are often specific for a pathogen race and can be quickly overcome by the evolution of the pathogen. In parallel, susceptibility genes (S-genes) are important for pathogenesis, and loss of S-gene function confers increased resistance in several plants, such as rice, wheat, citrus and tomatoes. In this article, we present the mutation and screening of seven putative S-genes in potatoes, including two DMR6 potato homologues. Using a CRISPR/Cas9 system, which conferred co-expression of two guide RNAs, tetra-allelic deletion mutants were generated and resistance against late blight was assayed in the plants. Functional knockouts of StDND1, StCHL1, and DMG400000582 (StDMR6-1) generated potatoes with increased resistance against late blight. Plants mutated in StDND1 showed pleiotropic effects, whereas StDMR6-1 and StCHL1 mutated plants did not exhibit any growth phenotype, making them good candidates for further agricultural studies. Additionally, we showed that DMG401026923 (here denoted StDMR6-2) knockout mutants did not demonstrate any increased late blight resistance, but exhibited a growth phenotype, indicating that StDMR6-1 and StDMR6-2 have different functions. To the best of our knowledge, this is the first report on the mutation and screening of putative S-genes in potatoes, including two DMR6 potato homologues.
Databáze: MEDLINE
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