Post-mortem molecular investigations of SARS-CoV-2 in an unexpected death of a recent kidney transplant recipient.

Autor: Simms EL; Department of Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada., Chung H; Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, Canada., Oberding L; Department of Pathology and Laboratory Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada., Muruve DA; Department of Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.; Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, Canada., McDonald B; Department of Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.; Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, Canada.; Department of Critical Care Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada., Bromley A; Department of Pathology and Laboratory Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada., Pillai DR; Department of Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.; Department of Pathology and Laboratory Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada., Chun J; Department of Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.; Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, Canada.
Jazyk: angličtina
Zdroj: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons [Am J Transplant] 2021 Jul; Vol. 21 (7), pp. 2590-2595. Date of Electronic Publication: 2021 Mar 15.
DOI: 10.1111/ajt.16549
Abstrakt: Solid organ transplant recipients are vulnerable to severe infection during induction therapy. We report a case of a 67-year-old male who died unexpectedly 10 days after receiving a kidney transplant on February 10, 2020. There was no clear cause of death, but COVID-19 was considered retrospectively, as the death occurred shortly after the first confirmed case of COVID-19 in Canada. We confirmed the presence of SARS-CoV-2 components in the renal allograft and native lung tissue using immunohistochemistry for SARS-CoV-2 spike protein and RNA scope in situ hybridization for SARS-CoV-2 RNA. Results were reaffirmed with the Food and Drug Administration Emergency Use Authorization approved Bio-Rad SARS-CoV-2 digital droplet PCR for the kidney specimen. Our case highlights the importance of patient autopsies in an unfolding global pandemic and demonstrates the utility of molecular assays to diagnose SARS-CoV-2 post-mortem. SARS-CoV-2 infection during induction therapy may portend a fatal clinical outcome. We also suggest COVID-19 may be transmittable via renal transplant.
(© 2021 The American Society of Transplantation and the American Society of Transplant Surgeons.)
Databáze: MEDLINE
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