A multiscale analysis of early flower development in Arabidopsis provides an integrated view of molecular regulation and growth control.

Autor:	Refahi Y; The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK; Laboratoire RDP, Université de Lyon 1, ENS-Lyon, INRAE, CNRS, UCBL, 69364 Lyon, France; Université de Reims Champagne Ardenne, INRAE, FARE, UMR A 614, 51097 Reims, France. Electronic address: yassin.refahi@inrae.fr., Zardilis A; The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK., Michelin G; Université Côte d'Azur, Inria, Sophia Antipolis, CNRS, I3S, France., Wightman R; The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK., Leggio B; Laboratoire RDP, Université de Lyon 1, ENS-Lyon, INRAE, CNRS, UCBL, 69364 Lyon, France., Legrand J; Laboratoire RDP, Université de Lyon 1, ENS-Lyon, INRAE, CNRS, UCBL, 69364 Lyon, France., Faure E; LIRMM, Univ Montpellier, CNRS, Montpellier, France., Vachez L; Laboratoire RDP, Université de Lyon 1, ENS-Lyon, INRAE, CNRS, UCBL, 69364 Lyon, France., Armezzani A; Laboratoire RDP, Université de Lyon 1, ENS-Lyon, INRAE, CNRS, UCBL, 69364 Lyon, France., Risson AE; Laboratoire RDP, Université de Lyon 1, ENS-Lyon, INRAE, CNRS, UCBL, 69364 Lyon, France., Zhao F; Laboratoire RDP, Université de Lyon 1, ENS-Lyon, INRAE, CNRS, UCBL, 69364 Lyon, France., Das P; Laboratoire RDP, Université de Lyon 1, ENS-Lyon, INRAE, CNRS, UCBL, 69364 Lyon, France., Prunet N; Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA., Meyerowitz EM; The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK; Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA; Howard Hughes Medical Institute and Division of Biology and Biological Engineering 156-29, California Institute of Technology, Pasadena, CA 91125, USA., Godin C; Laboratoire RDP, Université de Lyon 1, ENS-Lyon, INRAE, CNRS, UCBL, 69364 Lyon, France., Malandain G; Université Côte d'Azur, Inria, Sophia Antipolis, CNRS, I3S, France., Jönsson H; The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK; Computational Biology and Biological Physics, Lund University, Sölvegatan 14A, 223 62 Lund, Sweden; Department of Applied Mathematics and Theoretical Physics (DAMTP), University of Cambridge, Cambridge, UK. Electronic address: henrik.jonsson@slcu.cam.ac.uk., Traas J; Laboratoire RDP, Université de Lyon 1, ENS-Lyon, INRAE, CNRS, UCBL, 69364 Lyon, France. Electronic address: jan.traas@ens-lyon.fr.
Jazyk:	angličtina
Zdroj:	Developmental cell [Dev Cell] 2021 Feb 22; Vol. 56 (4), pp. 540-556.e8.
DOI:	10.1016/j.devcel.2021.01.019
Abstrakt:	We have analyzed the link between the gene regulation and growth during the early stages of flower development in Arabidopsis. Starting from time-lapse images, we generated a 4D atlas of early flower development, including cell lineage, cellular growth rates, and the expression patterns of regulatory genes. This information was introduced in MorphoNet, a web-based platform. Using computational models, we found that the literature-based molecular network only explained a minority of the gene expression patterns. This was substantially improved by adding regulatory hypotheses for individual genes. Correlating growth with the combinatorial expression of multiple regulators led to a set of hypotheses for the action of individual genes in morphogenesis. This identified the central factor LEAFY as a potential regulator of heterogeneous growth, which was supported by quantifying growth patterns in a leafy mutant. By providing an integrated view, this atlas should represent a fundamental step toward mechanistic models of flower development. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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