A gene-for-gene interaction involving a 'late' effector contributes to quantitative resistance to the stem canker disease in Brassica napus.

Autor: Jiquel A; INRAE, AgroParisTech, UMR BIOGER, Université Paris-Saclay, Avenue Lucien Brétignières, BP 01, Thiverval-Grignon, F-78850, France.; Euralis Semences, 6 Chemin des Panedautes, Mondonville, 31700, France., Gervais J; INRAE, AgroParisTech, UMR BIOGER, Université Paris-Saclay, Avenue Lucien Brétignières, BP 01, Thiverval-Grignon, F-78850, France., Geistodt-Kiener A; INRAE, AgroParisTech, UMR BIOGER, Université Paris-Saclay, Avenue Lucien Brétignières, BP 01, Thiverval-Grignon, F-78850, France.; Université Paris-Saclay, Route de l'Orme aux Merisiers, Saint-Aubin, 91190, France., Delourme R; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Gay EJ; INRAE, AgroParisTech, UMR BIOGER, Université Paris-Saclay, Avenue Lucien Brétignières, BP 01, Thiverval-Grignon, F-78850, France.; Université Paris-Saclay, Route de l'Orme aux Merisiers, Saint-Aubin, 91190, France., Ollivier B; INRAE, AgroParisTech, UMR BIOGER, Université Paris-Saclay, Avenue Lucien Brétignières, BP 01, Thiverval-Grignon, F-78850, France., Fudal I; INRAE, AgroParisTech, UMR BIOGER, Université Paris-Saclay, Avenue Lucien Brétignières, BP 01, Thiverval-Grignon, F-78850, France., Faure S; Innolea, 6 Chemin des Panedautes, Mondonville, 31700, France., Balesdent MH; INRAE, AgroParisTech, UMR BIOGER, Université Paris-Saclay, Avenue Lucien Brétignières, BP 01, Thiverval-Grignon, F-78850, France., Rouxel T; INRAE, AgroParisTech, UMR BIOGER, Université Paris-Saclay, Avenue Lucien Brétignières, BP 01, Thiverval-Grignon, F-78850, France.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2021 Aug; Vol. 231 (4), pp. 1510-1524. Date of Electronic Publication: 2021 Apr 02.
DOI: 10.1111/nph.17292
Abstrakt: The control of stem canker disease of Brassica napus (rapeseed), caused by the fungus Leptosphaeria maculans is based largely on plant genetic resistance: single-gene specific resistance (Rlm genes) or quantitative, polygenic, adult-stage resistance. Our working hypothesis was that quantitative resistance partly obeys the gene-for-gene model, with resistance genes 'recognizing' fungal effectors expressed during late systemic colonization. Five LmSTEE (stem-expressed effector) genes were selected and placed under the control of the AvrLm4-7 promoter, an effector gene highly expressed at the cotyledon stage of infection, for miniaturized cotyledon inoculation test screening of a gene pool of 204 rapeseed genotypes. We identified a rapeseed genotype, 'Yudal', expressing hypersensitive response to LmSTEE98. The LmSTEE98-RlmSTEE98 interaction was further validated by inactivation of the LmSTEE98 gene with a CRISPR-Cas9 approach. Isolates with mutated versions of LmSTEE98 induced more severe stem symptoms than the wild-type isolate in 'Yudal'. This single-gene resistance was mapped in a 0.6 cM interval of the 'Darmor_bzh' × 'Yudal' genetic map. One typical gene-for-gene interaction contributes partly to quantitative resistance when L. maculans colonizes the stems of rapeseed. With numerous other effectors specific to stem colonization, our study provides a new route for resistance gene discovery, elucidation of quantitative resistance mechanisms and selection for durable resistance.
(© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.)
Databáze: MEDLINE
Externí odkaz: