Glucagon-Producing Cell Expansion in Wistar Rats. Changes to Islet Architecture After Sleeve Gastrectomy.

Autor: Bancalero-delosReyes J; Servicio Extremeño de Salud, Complejo Hospitalario de Badajoz, Badajoz, Spain., Camacho-Ramírez A; Surgery Unit, Puerta del Mar University Hospital, University of Cadiz, Cadiz, Spain.; Asociación Gaditana de Apoyo al Investigador (AGAI), Cadiz, Spain.; Institute for Biomedical Science Research and Innovation (INIBICA), University of Cadiz, Cadiz, Spain., Fernández-Vivero J; Department of Human Anatomy and Embryology, Faculty of Medicine, University of Cadiz, Cadiz, Spain., Ribelles-García A; Department of Human Anatomy and Embryology, Faculty of Medicine, University of Cadiz, Cadiz, Spain.; Sustainable Social Development Research Institute (INDESS), University of Cadiz, Cadiz, Spain., Macías-Rodríguez M; Digestive Service, Puerta del Mar University Hospital, University of Cadiz, Cadiz, Spain., Almorza-Gomar D; Operative Statistic and Research Department, University of Cadiz, Cadiz, Spain., Carrasco-Molinillo C; Department of Human Anatomy and Embryology, Faculty of Medicine, University of Cadiz, Cadiz, Spain., Mayo-Ossorio MÁ; Surgery Unit, Puerta del Mar University Hospital, University of Cadiz, Cadiz, Spain.; Institute for Biomedical Science Research and Innovation (INIBICA), University of Cadiz, Cadiz, Spain., Prada-Oliveira JA; Asociación Gaditana de Apoyo al Investigador (AGAI), Cadiz, Spain. arturo.prada@uca.es.; Institute for Biomedical Science Research and Innovation (INIBICA), University of Cadiz, Cadiz, Spain. arturo.prada@uca.es.; Department of Human Anatomy and Embryology, Faculty of Medicine, University of Cadiz, Cadiz, Spain. arturo.prada@uca.es., Perez-Arana GM; Asociación Gaditana de Apoyo al Investigador (AGAI), Cadiz, Spain. gonzalo.perez@uca.es.; Institute for Biomedical Science Research and Innovation (INIBICA), University of Cadiz, Cadiz, Spain. gonzalo.perez@uca.es.; Department of Human Anatomy and Embryology, Faculty of Medicine, University of Cadiz, Cadiz, Spain. gonzalo.perez@uca.es.
Jazyk: angličtina
Zdroj: Obesity surgery [Obes Surg] 2021 May; Vol. 31 (5), pp. 2241-2249. Date of Electronic Publication: 2021 Feb 22.
DOI: 10.1007/s11695-021-05264-6
Abstrakt: Purpose: Many studies about bariatric surgery have analyzed the effect of sleeve gastrectomy (SG) on glucose improvement, beta-cell mass, and islet size modification. The effects of SG on the other endocrine cells of the pancreas, such as the alpha-cell population, and their regulatory mechanisms remain less studied.
Materials and Methods: We focused our work on the changes in the alpha-cell population after SG in a healthy model of Wistar rats. We measured alpha-cell mass, glucose tolerance, and insulin release after oral glucose tolerance tests and plasma glucagon secretion patterns after insulin infusion. Three Wistar rat groups were employed: SG-operated, surgical control (Sham), and fasting control.
Results: The results obtained showed significant increases in the alpha-cell population after SG. The result was an increase in beta-cell transdifferentiation; it was shown by some expressed molecules (the loss of expression of Pdx-1 and the increase in Arx and Pax6 cells/mm 2 of islet). The serum results were enhanced plasma glucagon secretion pattern after insulin infusion assays and normal glucose tolerance and insulin release after OGTT.
Conclusion: We concluded that SG leads to an expansion of the alpha-cell population, at expense of beta-cell; this expansion of alpha-cells is related to transdifferentiation. Plasma glucose level was not affected due to an increased glucagon response.
Databáze: MEDLINE
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