Loss of Arabidopsis matrix metalloproteinase-5 affects root development and root bacterial communities during drought stress.

Autor: Mishra LS; Department of Chemistry, Umeå University, Umeå, Sweden., Kim SY; Department of Chemistry, Umeå University, Umeå, Sweden.; Department of Plant Breeding, Swedish University of Agricultural Sciences, Uppsala, Sweden., Caddell DF; Department of Plant and Microbial Biology, University of California, Berkeley, California, USA.; US Department of Agriculture-Agricultural Research Service, Plant Gene Expression Center, Albany, California, USA., Coleman-Derr D; Department of Plant and Microbial Biology, University of California, Berkeley, California, USA.; US Department of Agriculture-Agricultural Research Service, Plant Gene Expression Center, Albany, California, USA., Funk C; Department of Chemistry, Umeå University, Umeå, Sweden.
Jazyk: angličtina
Zdroj: Physiologia plantarum [Physiol Plant] 2021 Jun; Vol. 172 (2), pp. 1045-1058. Date of Electronic Publication: 2020 Dec 16.
DOI: 10.1111/ppl.13299
Abstrakt: Matrix metalloproteinases (MMPs) are zinc-dependent endo-peptidases that in mammals are known to be involved in remodeling the extracellular matrix (ECM) in developmental and pathological processes. In this study, we report At5-MMP of Arabidopsis thaliana to be important for root development and root bacterial communities. At5-MMP is mainly localized in the root vasculature and lateral root, an At5-MMP T-DNA insertion mutant (mmp5 KO) showed reduced root growth and a lower number of root apexes, causing reduced water uptake from the soil. Subsequently, mmp5 KO is sensitive to drought stress. Inhibited auxin transport was accompanied with resistance to indole-3-acetic acid (IAA), 2, 4-dichlorophenoxyacetic acid (2, 4-D), and 1-naphthaleneacetic acid (NAA). The content of endogenous abscisic acid (ABA) was lower in roots of mmp5 KO than in wild type. Genes responsive to ABA as well as genes encoding enzymes of the proline biosynthesis were expressed to a lower extent in mmp5 KO than in wild type. Moreover, drought stress modulated root-associated bacterial communities of mmp5 KO: the number of Actinobacteria increased. Therefore, At5-MMP modulates auxin/ABA signaling rendering the plant sensitive to drought stress and recruiting differential root bacterial communities.
(© 2020 The Authors. Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society.)
Databáze: MEDLINE
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