Human glutathione peroxidase codon 198 variant increases nasopharyngeal carcinoma risk and progression.

Autor: Laribi A; Laboratory of Molecular Immuno‑Oncology, Faculty of Medicine of Monastir, University of Monastir, 5019, Monastir, Tunisia., Aouf S; Laboratory of Molecular Immuno‑Oncology, Faculty of Medicine of Monastir, University of Monastir, 5019, Monastir, Tunisia., Gabbouj S; Laboratory of Molecular Immuno‑Oncology, Faculty of Medicine of Monastir, University of Monastir, 5019, Monastir, Tunisia., Bouaouinaa N; Laboratory of Molecular Immuno‑Oncology, Faculty of Medicine of Monastir, University of Monastir, 5019, Monastir, Tunisia.; Department of Cancerology and Radiotherapy, Farhat Hached University Hospital, Monastir, Tunisia., Zakhama A; Laboratory of Molecular Immuno‑Oncology, Faculty of Medicine of Monastir, University of Monastir, 5019, Monastir, Tunisia.; Department of Anatomy and Pathologic Cytology, Fattouma Bourguiba University Hospital, Monastir, Tunisia., Harizi H; Laboratory of Molecular Immuno‑Oncology, Faculty of Medicine of Monastir, University of Monastir, 5019, Monastir, Tunisia. hharizi@hotmail.fr.; Faculty of Dental Medicine, University of Monastir, of Monastir, 5019, Monastir, Tunisia. hharizi@hotmail.fr.
Jazyk: angličtina
Zdroj: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery [Eur Arch Otorhinolaryngol] 2021 Oct; Vol. 278 (10), pp. 4027-4034. Date of Electronic Publication: 2021 Feb 22.
DOI: 10.1007/s00405-021-06628-5
Abstrakt: Purpose: Glutathione peroxidase 1 (GPx-1) is a selenium-dependent detoxifying enzyme involved in the protection of cells against oxidative damage. Some genetic association studies reported significant associations between GPx-1 Pro198Leu variant and carcinogenesis across different populations; however, the impact of this variant on nasopharyngeal carcinoma (NPC) has not been explored. Therefore, the present study was planned to evaluate the potential involvement of the GPx-1 Pro198Leu variant and plasma GPx activity in the risk of developing NPC in a Tunisian population.
Methods: The GPx-1 Pro198Leu genotype was determined in 327 NPC patients and 150 healthy controls by the RFLP-PCR analysis. The correlation between the GPx-1 variant and the clinicopathological parameters was examined. GPx activity was assessed in the plasma of 119 NPC patients and 58 healthy control subjects and according to GPx-1 genotypes and clinicopathological characteristics of NPC patients.
Results: A significant association was found between GPx-1 Pro198Leu variant and NPC risk in a Tunisian population. The allelic frequencies of Pro and Leu alleles were 32% versus 68% and 41% versus 59% in NPC cases and controls, respectively. Thus, the minor 198 Leu allele increased significantly in NPC patients and appeared as a potential risk factor for NPC occurrence (OR = 1.48, CI 95% = 1.14-1.91, p = 0.002). The plasma GPx activity was significantly higher in NPC patients than in controls (p = 0.03). According to the clinicopathological characteristics of NPC patients, GPx activity decreased significantly in patients with lymph node metastasis (p = 0.004).
Conclusion: This is the first study showing a strong association between GPx-1 Pro198Leu genetic variant and NPC risk. GPx-1 Pro198Leu variant increased the development of regional lymph node metastasis. Plasma GPx activity was higher in NPC patients. Thus, GPx-1 gene could be considered as a determinant factor influencing NPC risk and progression.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
Databáze: MEDLINE
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