Clinical trials optimizing investigator and self-collection of buccal cells for RNA yield.

Autor: Geiger JL; Department of Hematology and Medical Oncology Taussig Cancer Institute, Cleveland Clinic Cleveland Ohio USA., Cedars ED; Department of Otolaryngology-Head and Neck Surgery University of California San Francisco San Francisco California USA., Zang Y; Department of Medicine University of Pittsburgh School of Medicine Pittsburgh Pennsylvania USA., Normolle DP; Department of Biostatistics University of Pittsburgh Graduate School of Public Health Pittsburgh Pennsylvania USA., Li H; Department of Otolaryngology-Head and Neck Surgery University of California San Francisco San Francisco California USA., Grandis JR; Department of Otolaryngology-Head and Neck Surgery University of California San Francisco San Francisco California USA., Centuori S; Department of Medicine, Division of Hematology/Oncology University of Arizona College of Medicine Tucson Arizona USA., Johnson DE; Department of Otolaryngology-Head and Neck Surgery University of California San Francisco San Francisco California USA., Bauman JE; Department of Medicine, Division of Hematology/Oncology University of Arizona College of Medicine Tucson Arizona USA.
Jazyk: angličtina
Zdroj: Laryngoscope investigative otolaryngology [Laryngoscope Investig Otolaryngol] 2021 Jan 18; Vol. 6 (1), pp. 116-121. Date of Electronic Publication: 2021 Jan 18 (Print Publication: 2021).
DOI: 10.1002/lio2.516
Abstrakt: Objective: Buccal cells are an ideal surrogate tissue for studying biologic effects of carcinogens or drugs, however inherent fragility and salivary RNAses limit RNA yield. We conducted healthy volunteer trials to optimize collection conditions.
Methods: We conducted: (a) a single-arm crossover study evaluating four test conditions on RNA yield by buccal cytobrush; (b) a single-arm prospective study evaluating RNA yield by investigator vs self-collection.
Results: Antecedent toothbrushing, time of day, and number of cytobrush strokes did not significantly impact RNA yield. RNA yield was doubled by using 2 vs 1 cytobrush per buccal surface ( P = .0054). Self-collection of buccal cells for RNA was feasible; 36 of 50 (72%) samples passed quality control.
Conclusion: RNA yield was doubled by using two cytobrushes per buccal surface. Healthy volunteers can self-collect sufficient buccal RNA for gene expression studies. Techniques from these pragmatic trials could enhance availability of a limited tissue for serial biomarker measurements.
Level of Evidence: 1b-Prognosis Study (Individual prospective cohort study).
Competing Interests: Authors report no conflict of interest for the presented work.
(© 2021 The Authors. Laryngoscope Investigative Otolaryngology published by Wiley Periodicals LLC. on behalf of The Triological Society.)
Databáze: MEDLINE
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