Pollen Streptomyces Produce Antibiotic That Inhibits the Honey Bee Pathogen Paenibacillus larvae .

Autor:	Grubbs KJ; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, United States.; Department of Cellular and Molecular Pathology, University of Wisconsin-Madison, Madison, WI, United States., May DS; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, United States., Sardina JA; Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI, United States., Dermenjian RK; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, United States., Wyche TP; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, United States., Pinto-Tomás AA; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, United States., Clardy J; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, United States., Currie CR; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2021 Feb 04; Vol. 12, pp. 632637. Date of Electronic Publication: 2021 Feb 04 (Print Publication: 2021).
DOI:	10.3389/fmicb.2021.632637
Abstrakt:	Humans use natural products to treat disease; similarly, some insects use natural products produced by Actinobacteria to combat infectious pathogens. Honey bees, Apis mellifera , are ecologically and economically important for their critical role as plant pollinators and are host to diverse and potentially virulent pathogens that threaten hive health. Here, we provide evidence that Actinobacteria that can suppress pathogenic microbes are associated with A. mellifera . We show through culture-dependent approaches that Actinobacteria in the genus Streptomyces are commonly isolated from foraging bees, and especially common in pollen stores. One strain, isolated from pollen stores, exhibited pronounced inhibitory activity against Paenibacillus larvae , the causative agent of American foulbrood. Bioassay-guided HPLC fractionation, followed by NMR and mass spectrometry, identified the known macrocyclic polyene lactam, piceamycin that was responsible for this activity. Further, we show that in its purified form, piceamycin has potent inhibitory activity toward P. larvae . Our results suggest that honey bees may use pollen-derived Actinobacteria and their associated small molecules to mediate colony health. Given the importance of honey bees to modern agriculture and their heightened susceptibility to disease, the discovery and development of antibiotic compounds from hives could serve as an important strategy in supporting disease management within apiaries. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Grubbs, May, Sardina, Dermenjian, Wyche, Pinto-Tomás, Clardy and Currie.)
Databáze:	MEDLINE
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