Antiseizure potential of peptides from the venom of social wasp Chartergellus communis against chemically-induced seizures.

Autor: Lopes KS; Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília, Brazil., Quintanilha MVT; Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília, Brazil., de Souza ACB; Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília, Brazil., Zamudio-Zuñiga F; Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Morelos, Mexico., Possani LD; Department of Molecular Medicine and Bioprocesses, Institute of Biotechnology, National Autonomous University of Mexico, Morelos, Mexico., Mortari MR; Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília, Brazil. Electronic address: mmortari@unb.br.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2021 Apr 30; Vol. 194, pp. 23-36. Date of Electronic Publication: 2021 Feb 19.
DOI: 10.1016/j.toxicon.2021.02.009
Abstrakt: Epilepsy is one of the most common neurological diseases in the world. The objective of this research was to investigate a new peptide from the venom of the social wasp Chartergellus communis useful to the study or pharmacotherapy of epilepsy. The wasps were collected, and their venom was extracted. Afterward, the steps of fractionation, sequencing, and identification were carried out to obtain four peptides. These molecules were synthesized for behavioral evaluation tests and electroencephalographic assays to determine their antiseizure potential (induction of acute seizures using the chemical compounds, pentylenetetrazole - PTZ, and pilocarpine - PILO) and analysis of neuropharmacological profile (general spontaneous activity and alteration in motor coordination). Chartergellus-CP1 (i.c.v. - 3.0 μg/animal) caused beneficial alterations in some of the parameters evaluated in both models: PTZ (latency and duration of maximum seizures) and PILO (latency and duration of, and protection against, maximum seizures, and reduction of the median of the seizure scores. When evaluated in 3 doses in the seizure model induced by PILO, the dose of 3.0 μg/animal protected the animals against seizures, with an estimated ED 50 of 1.49 μg/animal. Electroencephalographic evaluation of Chartergellus-CP1 showed an improvement in latency, quantity, and percentage of protection against generalized electroencephalographic seizures in the PILO model. Further, Chartergellus-CP1 did not cause adverse effects on general spontaneous activity and motor coordination of animals. This study demonstrated how compounds isolated from wasps' venom may be important resources in the search for new drugs. Such compounds can be considered valuable therapeutic and biotechnological tools for the study and future treatment of epileptic disorders. In this context, a peptide that is potentially useful for epilepsy pharmacotherapy was identified in the venom of C. communis.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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