Serum Helicobacter pylori antibody reactivity in seven Asian countries using an automated latex aggregation turbidity assay.

Autor: Akada J; Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan., Tshibangu-Kabamba E; Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan., Tuan VP; Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan.; Department of Endoscopy, Cho Ray Hospital, Ho Chi Minh City, Vietnam., Kurogi S; Clinical Laboratory Center, Oita University Hospital, Yufu, Japan.; Department of Molecular Pathology, Faculty of Medicine, Oita University, Yufu, Japan., Matsuo Y; Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan., Ansari S; Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan., Doohan D; Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan.; Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia., Phuc BH; Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan., Subsomwong P; Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan., Waskito LA; Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan.; Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia., Binh TT; Department of Endoscopy, Cho Ray Hospital, Ho Chi Minh City, Vietnam., Nguyen LT; Department of Hepatogastroenterology, 108 Military Central Hospital, Hanoi, Vietnam., Khien VV; Department of Hepatogastroenterology, 108 Military Central Hospital, Hanoi, Vietnam., Dung HDQ; Department of Endoscopy, Cho Ray Hospital, Ho Chi Minh City, Vietnam., Miftahussurur M; Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia.; Gastroentero-Hepatology Division, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Airlangga University, Surabaya, Indonesia., Syam AF; Division of Gastroenterology, Department of Intestinal Medicine, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia., Tshering L; Department of Surgery, Jigme Dorji Wangchuck National Referral Hospital, Thimphu, Bhutan., Vilaichone RK; Gastroenterology Unit, Digestive Diseases Research Center, Thammasat University Hospital, Khlong Luang, Thailand.; Department of Medicine, Chulabhorn International College of Medicine, Thammasat University Hospital, Khlong Luang, Thailand., Mahachai V; GI and Liver Center, Bangkok Medical Center, Bangkok, Thailand., Ratanachu-Ek T; Department of Surgery, Rajavithi Hospital, Bangkok, Thailand., Shrestha PK; Department of Gastroenterology, Maharajgunj Medical Campus, Tribhuvan University Teaching Hospital, Kathmandu, Nepal., Yee TT; Department of GI and HBP Surgery, No (2) Defense Service General Hospital, Nay Pyi Taw, Myanmar., Htet K; Department of GI and HBP Surgery, No (1) Defense Service General Hospital, Mingaladon, Myanmar., Aftab H; Department of Gastroenterology, Dhaka Medical Collage and Hospital, Dhaka, Bangladesh., Matsuhisa T; Department of Gastroenterology, Tama-Nagayama University Hospital, Nippon Medical School, Tama, Japan., Uchida T; Department of Molecular Pathology, Faculty of Medicine, Oita University, Yufu, Japan., Okimoto T; Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan., Mizukami K; Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan., Kodama M; Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan.; Faculty of Welfare and Health Science, Oita University, Yufu, Japan., Murakami K; Department of Gastroenterology, Faculty of Medicine, Oita University, Yufu, Japan., Takahashi N; Clinical Laboratory Center, Oita University Hospital, Yufu, Japan., Yamaoka Y; Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu, Japan.; Global Oita Medical Advanced Research Center for Health, Oita University, Yufu, Japan.; Department of Medicine, Gastroenterology and Hepatology Section, Baylor College of Medicine, Houston, Texas, USA.
Jazyk: angličtina
Zdroj: Journal of gastroenterology and hepatology [J Gastroenterol Hepatol] 2021 Aug; Vol. 36 (8), pp. 2198-2209. Date of Electronic Publication: 2021 Mar 09.
DOI: 10.1111/jgh.15467
Abstrakt: Background and Aim: To determine the application range of diagnostic kits utilizing anti-Helicobacter pylori antibody, we tested a newly developed latex aggregation turbidity assay (latex) and a conventional enzyme-linked immunosorbent assay (E-plate), both containing Japanese H. pylori protein lysates as antigens, using sera from seven Asian countries.
Methods: Serum samples (1797) were obtained, and standard H. pylori infection status and atrophy status were determined by culture and histology (immunohistochemistry) using gastric biopsy samples from the same individuals. The two tests (enzyme-linked immunosorbent assay and latex) were applied, and receiver operating characteristics analysis was performed.
Results: Area under the curve (AUC) from the receiver operating characteristic of E-plate and latex curves were almost the same and the highest in Vietnam. The latex AUC was slightly lower than the E-plate AUC in other countries, and the difference became statistically significant in Myanmar and then Bangladesh as the lowest. To consider past infection cases, atrophy was additionally evaluated. Most of the AUCs decreased using this atrophy-evaluated status; however, the difference between the two kits was not significant in each country, but the latex AUC was better using all samples. Practical cut-off values were 3.0 U/mL in the E-test and 3.5 U/mL in the latex test, to avoid missing gastric cancer patients to the greatest extent possible.
Conclusions: The kits were applicable in all countries, but new kits using regional H. pylori strains are recommended for Myanmar and Bangladesh. Use of a cut-off value lower than the best cut-off value is essential for screening gastric cancer patients.
(© 2021 Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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