Characterisation of the T-cell response to Ebola virus glycoprotein amongst survivors of the 2013-16 West Africa epidemic.

Autor: Tipton TRW; National Infection Service, Public Health England, Porton Down, Salisbury, UK. tom.tipton@phe.gov.uk., Hall Y; National Infection Service, Public Health England, Porton Down, Salisbury, UK., Bore JA; Center for Training and Research on Priority Diseases including Malaria in Guinea (CEFORPAG), Nongo, Conakry, Guinea., White A; National Infection Service, Public Health England, Porton Down, Salisbury, UK., Sibley LS; National Infection Service, Public Health England, Porton Down, Salisbury, UK., Sarfas C; National Infection Service, Public Health England, Porton Down, Salisbury, UK., Yuki Y; Basic Science Program, Frederick National Laboratory for Cancer Research in the Laboratory of Integrative Cancer Immunology, National Cancer Institute, Frederick, MD, USA., Martin M; Basic Science Program, Frederick National Laboratory for Cancer Research in the Laboratory of Integrative Cancer Immunology, National Cancer Institute, Frederick, MD, USA., Longet S; National Infection Service, Public Health England, Porton Down, Salisbury, UK., Mellors J; National Infection Service, Public Health England, Porton Down, Salisbury, UK., Ewer K; The Jenner Institute, Oxford, UK., Günther S; Bernhard Nocht Institute for Tropical Medicine, Hamburg, DE, Germany.; German Center for Infection Research (DZIF), Partner Site Hamburg-Lübeck-Börstel-Riems, Hamburg, DE, Germany., Carrington M; Basic Science Program, Frederick National Laboratory for Cancer Research in the Laboratory of Integrative Cancer Immunology, National Cancer Institute, Frederick, MD, USA.; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA, USA., Kondé MK; Center for Training and Research on Priority Diseases including Malaria in Guinea (CEFORPAG), Nongo, Conakry, Guinea., Carroll MW; National Infection Service, Public Health England, Porton Down, Salisbury, UK.; Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Feb 19; Vol. 12 (1), pp. 1153. Date of Electronic Publication: 2021 Feb 19.
DOI: 10.1038/s41467-021-21411-0
Abstrakt: Zaire ebolavirus (EBOV) is a highly pathogenic filovirus which can result in Ebola virus disease (EVD); a serious medical condition that presents as flu like symptoms but then often leads to more serious or fatal outcomes. The 2013-16 West Africa epidemic saw an unparalleled number of cases. Here we show characterisation and identification of T cell epitopes in surviving patients from Guinea to the EBOV glycoprotein. We perform interferon gamma (IFNγ) ELISpot using a glycoprotein peptide library to identify T cell epitopes and determine the CD4 + or CD8 + T cell component response. Additionally, we generate data on the T cell phenotype and measure polyfunctional cytokine secretion by these antigen specific cells. We show candidate peptides able to elicit a T cell response in EBOV survivors and provide inferred human leukocyte antigen (HLA) allele restriction. This data informs on the long-term T cell response to Ebola virus disease and highlights potentially important immunodominant peptides.
Databáze: MEDLINE
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