SUGAR-seq enables simultaneous detection of glycans, epitopes, and the transcriptome in single cells.

Autor:	Kearney CJ; Translational Haematology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia. ricky.johnstone@petermac.org conor.kearney@petermac.org jane.oliaro@petermac.org.; Sir Peter MacCallum Department of Oncology, University of Melbourne, Victoria 3010, Australia., Vervoort SJ; Translational Haematology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia.; Sir Peter MacCallum Department of Oncology, University of Melbourne, Victoria 3010, Australia., Ramsbottom KM; Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia., Todorovski I; Translational Haematology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia., Lelliott EJ; Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia., Zethoven M; Translational Haematology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia., Pijpers L; Translational Haematology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia., Martin BP; Translational Haematology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia., Semple T; Translational Haematology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia., Martelotto L; Centre for Cancer Research, University of Melbourne, Melbourne 3000, VIC, Australia., Trapani JA; Sir Peter MacCallum Department of Oncology, University of Melbourne, Victoria 3010, Australia.; Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia., Parish IA; Sir Peter MacCallum Department of Oncology, University of Melbourne, Victoria 3010, Australia.; Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia., Scott NE; Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne 3000, Australia., Oliaro J; Sir Peter MacCallum Department of Oncology, University of Melbourne, Victoria 3010, Australia. ricky.johnstone@petermac.org conor.kearney@petermac.org jane.oliaro@petermac.org.; Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia., Johnstone RW; Translational Haematology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia. ricky.johnstone@petermac.org conor.kearney@petermac.org jane.oliaro@petermac.org.; Sir Peter MacCallum Department of Oncology, University of Melbourne, Victoria 3010, Australia.; Cancer Therapeutics Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2021 Feb 19; Vol. 7 (8). Date of Electronic Publication: 2021 Feb 19 (Print Publication: 2021).
DOI:	10.1126/sciadv.abe3610
Abstrakt:	Multimodal single-cell RNA sequencing enables the precise mapping of transcriptional and phenotypic features of cellular differentiation states but does not allow for simultaneous integration of critical posttranslational modification data. Here, we describe SUrface-protein Glycan And RNA-seq (SUGAR-seq), a method that enables detection and analysis of N-linked glycosylation, extracellular epitopes, and the transcriptome at the single-cell level. Integrated SUGAR-seq and glycoproteome analysis identified tumor-infiltrating T cells with unique surface glycan properties that report their epigenetic and functional state. (Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
Databáze:	MEDLINE
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