| Autor: |
Orbán L; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore. orban.laszlo@georgikon.hu.; Frontline Fish Genomics Research Group, Department of Animal Sciences, Georgikon Campus, Szent István University, Keszthely, Hungary. orban.laszlo@georgikon.hu.; Centre for Comparative Genomics, Murdoch University, Murdoch, WA, Australia. orban.laszlo@georgikon.hu., Saju JM; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore., Tzung KW; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore.; Institute of Molecular and Cell Biology (IMCB), A*STAR, Singapore, Singapore., Liew WC; Reproductive Genomics Group, Temasek Life Sciences Laboratory, Singapore, Singapore. |
| Abstrakt: |
The regulation of reproduction in zebrafish, the prime model of fish research, is not fully understood. An efficient tool to gain a better understanding of this complicated process is utilization of severely sex-biased families or groups. Here, we describe a method for partial depletion of primordial germ cells (PGCs) that leads to eventual masculinization of zebrafish. The technique is based on injecting early embryos with diluted morpholino oligonucleotides that temporarily interfere with the production of Dead end (Dnd), an RNA-binding protein essential for PGC survival. In addition, we also propose the use of eviscerated trunk, as a suitable alternative for examining gonadal expression in juvenile zebrafish. |
