Sleep Deprivation Interferes with JAK/STAT Signaling Pathway and Myogenesis in the Masseter Muscle of Rats.

Autor:	Gomes Galvani M; Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil., Moreira Antunes HK; Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil., Monico-Neto M; Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil., Yujra VQ; Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil., Prado CM; Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil., Quintana HT; Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil., de Oliveira F; Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil., Ribeiro DA; Department of Biosciences, Federal University of São Paulo, UNIFESP, Santos, Brazil.
Jazyk:	angličtina
Zdroj:	Medical principles and practice : international journal of the Kuwait University, Health Science Centre [Med Princ Pract] 2021; Vol. 30 (3), pp. 253-261. Date of Electronic Publication: 2021 Feb 18.
DOI:	10.1159/000515307
Abstrakt:	Objectives: The aim of the study was to study the Janus kinase/tyrosine kinase-activated transduction factor (JAK/STAT) signaling pathway and myogenesis on the masseter muscle after sleep deprivation and to investigate the role of stress in this scenario. Subjects and Methods: A total of 18 male Wistar rats were divided into the following groups: control (n = 6): animals were not submitted to any procedures, and paradoxical sleep deprivation and vehicle (PSD + V; n = 6): animals were subjected to PSD for 96 h and (PSD + MET; n = 6): animals were subjected to PSD for 96 h with administration of metyrapone. Paradoxical sleep deprivation was performed by the modified multiple platforms method. Histopathological analysis, histomorphometry, and immunohistochemistry were performed. Results: The results showed the presence of inflammatory infiltrate in the PSD + V and PSD + MET groups and atrophy. Histomorphometry showed that the cellular profile area decreased, while cellular density increased in both experimental groups. Expression of p-STAT 3, MyoD, and MyoG increased in the PSD + V group, while the PSD + MET group showed increased expression of IL-6 and p-STAT 3. Conclusion: Our results suggest that sleep deprivation induces an inflammatory response and atrophy in the masseter muscle of rats. (© 2021 The Author(s) Published by S. Karger AG, Basel.)
Databáze:	MEDLINE
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