Glutathione S transferase T1 gene polymorphism and its promoter methylation are associated with breast cancer susceptibility in Egyptian women.

Autor: Raafat N; Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt., Ismail K; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia., Hawsawi NM; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia., Saber T; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia., Elsawy WH; Department of Clinical Oncology and Nuclear Medicine, Faculty of Medicine, Zagazig University, Egypt., Abdulmutaleb T; Ministry of Health, King Faisal Medical Complex Alpakistany, Taif, Saudi Arabia., Raafat A; General Surgery Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt., Gharib AF; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia.
Jazyk: angličtina
Zdroj: Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2022 Apr; Vol. 69 (2), pp. 526-533. Date of Electronic Publication: 2021 Mar 19.
DOI: 10.1002/bab.2129
Abstrakt: Background: Breast cancer (BC) is one of the leading causes of cancer mortality in women. Glutathione S-transferase (GSTT1) is involved in activation of detoxification reactions and catalysis of chemicals conjugation with glutathione. GSTT1 genotype is a limiting factor for some environmental diseases. Epigenetic changes have an essential role in BC through inappropriate interaction between genomic and environmental risk factors.
Aim: This study was directed to explore the association of BC risk with GSTT1 genetic variations and its methylation status in Egyptian women.
Design and Methods: This study included 100 healthy women as the control group and 100 patients were clinically and histologically diagnosed with breast cancer. All blood samples were used for genomic DNA extraction. GSTT1 genotyping was accomplished by multiplex PCR and methylation-specific PCR was used to analyze the GSTT1 promoter methylation status.
Results: Breast cancer patients showed significant incidence of null GSTT1 in relation to controls (p = 0.004). GSTT1 gene promoter methylation status showed significant difference between hypermethylated and unmethylated patients when compared with healthy subjects (p = 0.005). GSTT1 promoter methylation status was not significantly associated with null genotype. There was no significant association between GSTT1-null genotypes and BC stage in cases with or without family history, but for promotor methylation, there was significant association with stage III and IV breast cancer disease.
Conclusion: GSTT1 null genetic variant and promoter hypermethylation in the GSTT region of the gene may be considered as critical risk factors for BC in Egyptian women.
(© 2021 International Union of Biochemistry and Molecular Biology, Inc.)
Databáze: MEDLINE
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