Surface-specific washing-free immunosensor for time-resolved cortisol monitoring.

Autor: Safarian SM; Skolkovo Institute of Science and Technology, 3, Nobel Street, Moscow, 143025, Russia., Kusov PA; Skolkovo Institute of Science and Technology, 3, Nobel Street, Moscow, 143025, Russia., Kosolobov SS; Skolkovo Institute of Science and Technology, 3, Nobel Street, Moscow, 143025, Russia., Borzenkova OV; Skolkovo Institute of Science and Technology, 3, Nobel Street, Moscow, 143025, Russia., Khakimov AV; Skolkovo Institute of Science and Technology, 3, Nobel Street, Moscow, 143025, Russia., Kotelevtsev YV; Skolkovo Institute of Science and Technology, 3, Nobel Street, Moscow, 143025, Russia., Drachev VP; Skolkovo Institute of Science and Technology, 3, Nobel Street, Moscow, 143025, Russia; University of North Texas, Denton, TX, 76203, USA. Electronic address: V.drachev@skoltech.ru.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2021 Apr 01; Vol. 225, pp. 122070. Date of Electronic Publication: 2020 Dec 29.
DOI: 10.1016/j.talanta.2020.122070
Abstrakt: Cortisol is a steroid hormone that regulates a wide range of vital processes. Its level changes with diurnal rhythm and reacts to stress. Measurement of cortisol levels is still a complex multi-step process. A reversible washing-free registration method is required. Here we describe metal-enhanced fluorescence assay based on a displacement of a dye labeled BSA-cortisol conjugate from the immune complex immobilized on the golden islands by free cortisol. This competitive approach allows time-resolved monitoring of the fluorescent signal, surface-enhanced by the gold film, and provides the possibility of continuous real-time cortisol monitoring based on the implantable surface-enhanced immunosensor, which was not demonstrated so far even in vitro.
(Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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