An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution.

Autor:	Howard AG 4th; Department of BioSciences, Rice University, Houston, United States., Baker PA; Department of BioSciences, Rice University, Houston, United States., Ibarra-García-Padilla R; Department of BioSciences, Rice University, Houston, United States., Moore JA; Department of BioSciences, Rice University, Houston, United States., Rivas LJ; Department of BioSciences, Rice University, Houston, United States., Tallman JJ; Department of BioSciences, Rice University, Houston, United States., Singleton EW; Department of BioSciences, Rice University, Houston, United States., Westheimer JL; Department of BioSciences, Rice University, Houston, United States., Corteguera JA; Department of BioSciences, Rice University, Houston, United States., Uribe RA; Department of BioSciences, Rice University, Houston, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Feb 16; Vol. 10. Date of Electronic Publication: 2021 Feb 16.
DOI:	10.7554/eLife.60005
Abstrakt:	Neural crest cells (NCCs) are vertebrate stem cells that give rise to various cell types throughout the developing body in early life. Here, we utilized single-cell transcriptomic analyses to delineate NCC-derivatives along the posterior developing vertebrate, zebrafish, during the late embryonic to early larval stage, a period when NCCs are actively differentiating into distinct cellular lineages. We identified several major NCC/NCC-derived cell-types including mesenchyme, neural crest, neural, neuronal, glial, and pigment, from which we resolved over three dozen cellular subtypes. We dissected gene expression signatures of pigment progenitors delineating into chromatophore lineages, mesenchyme cells, and enteric NCCs transforming into enteric neurons. Global analysis of NCC derivatives revealed they were demarcated by combinatorial hox gene codes, with distinct profiles within neuronal cells. From these analyses, we present a comprehensive cell-type atlas that can be utilized as a valuable resource for further mechanistic and evolutionary investigations of NCC differentiation. Competing Interests: AH, PB, RI, JM, LR, JT, ES, JW, JC, RU No competing interests declared (© 2021, Howard et al.)
Databáze:	MEDLINE
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