Single-cell transcriptomic analysis of the adult mouse spinal cord reveals molecular diversity of autonomic and skeletal motor neurons.

Autor: Blum JA; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.; Stanford Neurosciences Graduate Program, Stanford University School of Medicine, Stanford, CA, USA., Klemm S; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA., Shadrach JL; Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA., Guttenplan KA; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.; Stanford Neurosciences Graduate Program, Stanford University School of Medicine, Stanford, CA, USA., Nakayama L; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA., Kathiria A; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA., Hoang PT; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.; Department of Neurology and Neurological Science, Stanford University School of Medicine, Stanford, CA, USA., Gautier O; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.; Stanford Neurosciences Graduate Program, Stanford University School of Medicine, Stanford, CA, USA., Kaltschmidt JA; Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA., Greenleaf WJ; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.; Department of Applied Physics, Stanford University, Stanford, CA, USA.; Chan Zuckerberg Biohub, San Francisco, CA, USA., Gitler AD; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA. agitler@stanford.edu.
Jazyk: angličtina
Zdroj: Nature neuroscience [Nat Neurosci] 2021 Apr; Vol. 24 (4), pp. 572-583. Date of Electronic Publication: 2021 Feb 15.
DOI: 10.1038/s41593-020-00795-0
Abstrakt: The spinal cord is a fascinating structure that is responsible for coordinating movement in vertebrates. Spinal motor neurons control muscle activity by transmitting signals from the spinal cord to diverse peripheral targets. In this study, we profiled 43,890 single-nucleus transcriptomes from the adult mouse spinal cord using fluorescence-activated nuclei sorting to enrich for motor neuron nuclei. We identified 16 sympathetic motor neuron clusters, which are distinguishable by spatial localization and expression of neuromodulatory signaling genes. We found surprising skeletal motor neuron heterogeneity in the adult spinal cord, including transcriptional differences that correlate with electrophysiologically and spatially distinct motor pools. We also provide evidence for a novel transcriptional subpopulation of skeletal motor neuron (γ*). Collectively, these data provide a single-cell transcriptional atlas ( http://spinalcordatlas.org ) for investigating the organizing molecular logic of adult motor neuron diversity, as well as the cellular and molecular basis of motor neuron function in health and disease.
Databáze: MEDLINE
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