BRCA1/BARD1 site-specific ubiquitylation of nucleosomal H2A is directed by BARD1.

Autor: Witus SR; Department of Biochemistry, University of Washington, Seattle, WA, USA., Burrell AL; Department of Biochemistry, University of Washington, Seattle, WA, USA., Farrell DP; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Institute for Protein Design, University of Washington, Seattle, WA, USA., Kang J; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Department of Chemistry, University of Washington, Seattle, WA, USA., Wang M; Department of Biochemistry and Structural Biology, University of Texas Health San Antonio, San Antonio, TX, USA., Hansen JM; Department of Biochemistry, University of Washington, Seattle, WA, USA., Pravat A; Department of Biochemistry, University of Washington, Seattle, WA, USA., Tuttle LM; Department of Biochemistry, University of Washington, Seattle, WA, USA., Stewart MD; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Department of Biology, Texas Christian University, Fort Worth, TX, USA., Brzovic PS; Department of Biochemistry, University of Washington, Seattle, WA, USA., Chatterjee C; Department of Chemistry, University of Washington, Seattle, WA, USA., Zhao W; Department of Biochemistry and Structural Biology, University of Texas Health San Antonio, San Antonio, TX, USA., DiMaio F; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Institute for Protein Design, University of Washington, Seattle, WA, USA., Kollman JM; Department of Biochemistry, University of Washington, Seattle, WA, USA., Klevit RE; Department of Biochemistry, University of Washington, Seattle, WA, USA. klevit@uw.edu.
Jazyk: angličtina
Zdroj: Nature structural & molecular biology [Nat Struct Mol Biol] 2021 Mar; Vol. 28 (3), pp. 268-277. Date of Electronic Publication: 2021 Feb 15.
DOI: 10.1038/s41594-020-00556-4
Abstrakt: Mutations in the E3 ubiquitin ligase RING domains of BRCA1/BARD1 predispose carriers to breast and ovarian cancers. We present the structure of the BRCA1/BARD1 RING heterodimer with the E2 enzyme UbcH5c bound to its cellular target, the nucleosome, along with biochemical data that explain how the complex selectively ubiquitylates lysines 125, 127 and 129 in the flexible C-terminal tail of H2A in a fully human system. The structure reveals that a novel BARD1-histone interface couples to a repositioning of UbcH5c compared to the structurally similar PRC1 E3 ligase Ring1b/Bmi1 that ubiquitylates H2A Lys119 in nucleosomes. This interface is sensitive to both H3 Lys79 methylation status and mutations found in individuals with cancer. Furthermore, NMR reveals an unexpected mode of E3-mediated substrate regulation through modulation of dynamics in the C-terminal tail of H2A. Our findings provide insight into how E3 ligases preferentially target nearby lysine residues in nucleosomes by a steric occlusion and distancing mechanism.
Databáze: MEDLINE
Externí odkaz: